A sensitive electrochemical immunosensing platform for the detection of Cronobacter sakazakii was developed using a graphene oxide/gold (GO/Au) composite. Transmission electron microscopy showed that the Au nanoparticles, with an average size of < 30 nm, were well dispersed on the GO surface. For the detection of C. sakazakii, a polyclonal anti-C. sakazakii antibody (IgG) was covalently immobilized to the Au nanoparticles on the surface of the GO/Au composite coated glassy carbon electrode (GCE). The electrochemical sensing performance of immunofunctionalized GCE was characterized by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, in pure culture there was a linear relationship between electrical signal and C. sakazakii levels over the range 2.0 × 10²–2.0 × 10⁷ cfu/mL (R² = 0.999), with a detection limit of 2.0 × 10¹ cfu/mL. The total analytical time was 15 min per sample. The C. sakazakii electrochemical immunosensing assay was able to successfully detect 2.0 × 10¹ cfu/mL of C. sakazakii in artificially contaminated powdered infant formula without any enrichment or pre-enrichment steps. Furthermore, the recovery rates of the C. sakazakii electrochemical immunosensing assay following spiking of powdered infant formula with different concentrations of C. sakazakii (cfu/mL) were 82.58% at 2.0 × 10¹ cfu/mL, 84.86% at 2.0 × 10² cfu/mL, and 95.40% at 2.0 × 10³ cfu/mL. The C. sakazakii electrochemical immunosensing assay had good selectivity, reproducibility, and reactivity compared with other Cronobacter spp. and/or pathogens belonging to other genera, indicating its significant potential in the clinical diagnosis of C. sakazakii.

使用氧化石墨烯/金（GO / Au）复合材料开发了一种用于检测阪崎肠杆菌的灵敏电化学免疫传感平台。透射电子显微镜显示平均尺寸小于30 nm的Au纳米颗粒很好地分散在GO表面。为了检测阪崎肠杆菌，将多克隆抗阪崎肠杆菌抗体（IgG）共价固定在GO / Au复合涂层玻碳电极（GCE）表面的Au纳米颗粒上。通过循环伏安法和差分脉冲伏安法表征了免疫功能化GCE的电化学传感性能。在最佳条件下，在纯培养中，电信号与阪崎肠杆菌水平在2.0×10 ² –2.0×10 ⁷ cfu / mL范围内（R ² = 0.999），检出限为2.0×10 ¹ cfu / mL。每个样品的总分析时间为15分钟。的C.阪崎电化学免疫测定法是能够成功地检测2.0×10 ¹ CFU / mL的C.阪崎肠杆菌在人工污染婴儿配方奶粉而没有任何富集或预先富集步骤。此外，加标浓度不同的阪崎肠杆菌（cfu / mL）的婴儿配方粉加标后，阪崎肠杆菌电化学免疫检测法的回收率为2.0×10为82.58％。^1个CFU /毫升，在2.0×10 84.86％² CFU / mL，并且在2.0×10 95.40％³ CFU /毫升。的C.阪崎电化学免疫测定法具有良好的选择性，重现性和反应性与其它相比阪属。和/或属于其他属的病原体，表明其在阪崎肠杆菌的临床诊断中具有重要的潜力。