Rotaviral gastroenteritis is associated with high rate of infant mortality and morbidity. Antirotaviral activity has been associated with some glycoproteins, such as immunoglobulins A (IgA), lactoferrin (LF), mucins and lactadherin of human milk. Although holder pasteurization (HoP, 63 °C for 30 min) is the treatment currently applied to human milk, it may lead to a decrease of its bioactive properties. The antirotaviral capacity of human milk showed to be mainly associated with the whey fraction, focusing on IgA and LF, with neutralizing values of 100, 100 and 62%, at 1 mg protein/mL, respectively. HoP reduced the antirotaviral activity of human whey, IgA and LF, 30, 98 and 60%, respectively. Interestingly, high temperature-short time (HTST) pasteurization at 75 °C for 20 s did not affect the antirotaviral activity of samples, while the highest HHP treatment at 600 MPa for 15 min only reduced the activity of human whey, IgA and LF, 9, 40 and 10%, respectively.

轮状肠胃炎与婴儿高死亡率和高发病率有关。抗旋转病毒活性与某些糖蛋白有关，例如人乳中的免疫球蛋白A（IgA），乳铁蛋白（LF），粘蛋白和乳黏附素。尽管目前已对持乳者进行巴氏杀菌（HoP，63°C，持续30分钟），但这可能会导致其生物活性下降。结果表明，人乳的抗旋转病毒能力主要与乳清部分有关，主要集中在IgA和LF上，在1 mg蛋白/ mL下的中和值分别为100％，100％和62％。HoP分别降低了人乳清，IgA和LF的抗旋转病毒活性，分别为30％，98％和60％。有趣的是，高温短时（HTST）在75°C下巴氏灭菌20 s不会影响样品的抗旋转病毒活性，