For the first time, rupture event scanning (REVS) procedure based on quartz crystal microbalance (QCM) and involving only mechanical action was used to determine the height of the energy barrier for dsDNA unwinding. Melting point was determined with the help of this procedure. To determine the thermodynamic parameters including enthalpy, DNA denaturation was represented as a unimolecular process. This allowed us to recover the energy profiles from the experimental data obtained by force measurements at different scanning times (reaction times) for different temperatures. The thus obtained results were compared with the data obtained with the help of another mechanical method, namely, atomic force microscopy. The mechanism of DNA unwinding in QCM-based experiments through the unzipping mode, as proposed by us in previous works, was confirmed. Thus, we demonstrated that REVS procedure may be used to assess the thermodynamic parameters of dsDNA unwinding.

中文翻译：

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借助机械方法确定DNA双螺旋展开的热力学参数

首次使用基于石英晶体微量天平（QCM）且仅涉及机械作用的破裂事件扫描（REVS）程序来确定dsDNA展开的能垒高度。借助该程序确定熔点。为了确定包括焓的热力学参数，将DNA变性表示为单分子过程。这使我们能够从通过在不同温度下不同扫描时间（反应时间）进行力测量获得的实验数据中恢复能量分布。将由此获得的结果与借助另一种机械方法即原子力显微镜获得的数据进行比较。正如我们在先前的工作中所提出的，在基于QCM的实验中通过解压缩模式解开DNA的机制得到了证实。因此，