This paper presents a rapid, sensitive and precise method for the determination of metaldehyde in human blood, using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry and high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry. Separation was performed with a Poroshell 120 EC-C18 column; 2.7 μm atrazine‑d5 (IS) and 200 mg NaCl were added to the blood sample. Proteins in human blood were precipitated using acetonitrile; the supernatant was then analyzed with the UHPLC-Q-TOF-MS or HPLC-QqQ-MS/MS system. The results of selectivity, linearity, accuracy, precision, limits of quantification, recovery, and matrix effects were sufficient to enable the measurement of metaldehyde in human blood samples. In addition, we proposed a fragmentation pathway involving ammonium adduct fragment ions for metaldehyde.

本文提供了一种快速，灵敏，精确的测定人血中乙醛的方法，该方法采用了超高效液相色谱与四极杆飞行时间串联质谱联用，以及高效液相色谱与三重四极杆串联质谱联用。用Poroshell 120 EC-C18色谱柱进行分离。将2.7μm阿特拉津-d5（IS）和200 mg NaCl添加到血液样本中。用乙腈沉淀人血中的蛋白质；然后用UHPLC-Q-TOF-MS或HPLC-QqQ-MS / MS系统分析上清液。选择性，线性，准确度，精密度，定量限，回收率和基质效应的结果足以进行人血样品中甲醛的测定。此外，