Clonostachys chloroleuca 67-1 (formerly C. rosea 67-1) is a potential biocontrol fungus active against various fungal plant pathogens. From transcriptome sequencing of 67-1 parasitizing sclerotia of Sclerotinia sclerotiorum, we identified the transcription factor-encoding gene crtf that is significantly up-regulated during mycoparasitism. Transcription factors are widely distributed in fungi and involved in multiple biological processes. However, their role and regulatory mechanisms in mycoparasitism remain poorly understood. In this study, the function of crtf during 67-1 mycoparasitism was verified through gene knockout and complementation. The results showed that deletion of crtf did not influence fungal morphological characteristics, but the ability of the Δcrtf mutant to parasitize sclerotia and suppress soybean Sclerotinia white mold in the greenhouse was markedly diminished compared with the wild type strain. The biocontrol activity of Δcrtf recovered wild type levels when complemented with a plasmid expressing the crtf gene. These findings suggest that crtf plays a crucial role in C. chloroleuca mycoparasitism and provide insight into the molecular mechanisms underlying C. chloroleuca mycoparasitism on plant pathogenic fungi.

Clonostachys greenleuca 67-1（以前称为C. Rosea 67-1）是一种潜在的生物防治真菌，对多种植物真菌病原体具有活性。通过对核盘菌67-1寄生菌核的转录组测序，我们鉴定出在菌核病寄生过程中显着上调的转录因子编码基因crtf 。转录因子广泛分布于真菌中并参与多种生物过程。然而，它们在菌寄生中的作用和调节机制仍然知之甚少。本研究通过基因敲除和互补验证了crtf在67-1菌寄生过程中的功能。结果表明， crtf缺失并不影响真菌形态特征，但Δcrtf突变体在温室中寄生菌核和抑制大豆菌核病白霉的能力较野生型菌株明显减弱。当与表达crtf基因的质粒互补时，Δ crtf的生物防治活性恢复了野生型水平。这些发现表明， crtf在绿鳖菌真菌寄生中发挥着至关重要的作用，并为了解绿鳖菌真菌寄生在植物病原真菌上的分子机制提供了见解。