Bisphenol A (BPA) may be a critical risk factor for metabolic disorders including obesity and diabetes. Sulforaphane is known to inhibit adipocyte differentiation and induce adipocyte lipolysis. This study sought to investigate the possible effects of sulforaphane on BPA-induced adipocyte differentiation in 3T3-L1 cells. During the preadipocytes differentiation process, sulforaphane suppressed the expression of early adipogenic factors, PPARγ and C/EBPα, induced by BPA in 3T3-L1 cells. Based on cell cycle analysis, sulforaphane mediated the inhibition of mitotic clonal expansion via cell cycle arrest at the G0/G1 phase. The compound inhibited cell cycle progression by suppressing cell cycle regulators such as cyclin D1 and pRb and by increasing p21 and p27, cell cycle inhibitors. Moreover, sulforaphane activated phosphorylation of AMPK and ACC and decreased that of ERK1/2 and Akt. Sulforaphane exerts inhibitory effects on BPA-induced adipocyte differentiation in 3T3-L1 cells. Sulforaphane could be adipogenic properties for improving environmental pollutants.

双酚A（BPA）可能是代谢性疾病（包括肥胖症和糖尿病）的关键危险因素。已知萝卜硫烷抑制脂肪细胞分化并诱导脂肪细胞脂解。这项研究试图调查萝卜硫烷对3T3-L1细胞中BPA诱导的脂肪细胞分化的可能影响。在前脂肪细胞分化过程中，萝卜硫烷抑制了BPA在3T3-L1细胞中诱导的早期成脂因子PPARγ和C /EBPα的表达。基于细胞周期分析，萝卜硫烷通过抑制G0 / G1期的细胞周期介导了有丝分裂克隆扩增的抑制作用。该化合物通过抑制细胞周期调节剂（如细胞周期蛋白D1和pRb）以及增加细胞周期抑制剂p21和p27来抑制细胞周期进程。而且，萝卜硫素激活了AMPK和ACC的磷酸化，并降低了ERK1 / 2和Akt的磷酸化。萝卜硫烷对BPA诱导的3T3-L1细胞中的脂肪细胞分化产生抑制作用。萝卜硫烷可能是改善环境污染物的成脂特性。