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Fabrication by Laser Irradiation in a Continuous Flow Jet of Carbon Quantum Dots for Fluorescence Imaging
ACS Omega ( IF 3.7 ) Pub Date : 2018-03-07 00:00:00 , DOI: 10.1021/acsomega.7b02082 Carlos Doñate-Buendia 1 , Rafael Torres-Mendieta 2 , Alexander Pyatenko 3 , Eva Falomir 4 , Mercedes Fernández-Alonso 1 , Gladys Mínguez-Vega 1
ACS Omega ( IF 3.7 ) Pub Date : 2018-03-07 00:00:00 , DOI: 10.1021/acsomega.7b02082 Carlos Doñate-Buendia 1 , Rafael Torres-Mendieta 2 , Alexander Pyatenko 3 , Eva Falomir 4 , Mercedes Fernández-Alonso 1 , Gladys Mínguez-Vega 1
Affiliation
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Fluorescent carbon quantum dots (CQDs) are synthesized by laser irradiation of carbon glassy particles suspended in polyethylene glycol 200 by two methods, a batch and a flow jet configuration. The flow jet configuration is carried out by the simple combination of common laboratory objects to construct a home-made passage reactor of continuous flow. Despite the simplicity of the system, the laser energy is better harvested by the carbon microparticles, improving the fabrication efficiency a 15% and enhancing the fluorescence of CQDs by an order of magnitude in comparison with the conventional batch. The flow jet-synthesized CQDs have a mean size of 3 nm and are used for fluorescent imaging of transparent healthy and cancer epithelial human cells. Complete internalization is observed with a short incubation time of 10 min without using any extra additive or processing of the cell culture. The CQDs are well fixed in the organelles of the cell even after its death; hence, this is a simple manner to keep the cell information for prolonged periods of time. Moreover, the integrated photostability of the CQDs internalized in in vitro cells is measured and it remains almost constant during at least 2 h, revealing their outstanding performance as fluorescent labels.
中文翻译：
碳量子点连续流动射流中激光照射的荧光成像制备
荧光碳量子点（CQD）是通过分批和射流构造两种方法通过激光照射悬浮在聚乙二醇200中的碳玻璃状颗粒而合成的。通过普通实验室对象的简单组合来进行射流配置，以构造出连续流动的自制通道反应器。尽管系统简单，但碳微粒可更好地收集激光能量，与常规批次相比，其制造效率提高了15％，CQD的荧光增强了一个数量级。射流合成的CQD的平均大小为3 nm，用于透明的健康和癌症上皮人细胞的荧光成像。在短短的10分钟孵育时间内即可观察到完全内在化，而无需使用任何额外的添加剂或处理细胞培养物。即使在细胞死亡后，CQD也能很好地固定在细胞的细胞器中。因此，这是保持小区信息长时间的简单方式。此外，测量了体外细胞内在化的CQD的综合光稳定性，并且在至少2 h内几乎保持恒定，从而揭示了其作为荧光标记的出色性能。
更新日期：2018-03-07
中文翻译：
碳量子点连续流动射流中激光照射的荧光成像制备
荧光碳量子点（CQD）是通过分批和射流构造两种方法通过激光照射悬浮在聚乙二醇200中的碳玻璃状颗粒而合成的。通过普通实验室对象的简单组合来进行射流配置，以构造出连续流动的自制通道反应器。尽管系统简单，但碳微粒可更好地收集激光能量，与常规批次相比，其制造效率提高了15％，CQD的荧光增强了一个数量级。射流合成的CQD的平均大小为3 nm，用于透明的健康和癌症上皮人细胞的荧光成像。在短短的10分钟孵育时间内即可观察到完全内在化，而无需使用任何额外的添加剂或处理细胞培养物。即使在细胞死亡后，CQD也能很好地固定在细胞的细胞器中。因此，这是保持小区信息长时间的简单方式。此外，测量了体外细胞内在化的CQD的综合光稳定性，并且在至少2 h内几乎保持恒定，从而揭示了其作为荧光标记的出色性能。
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