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Cholesterol-Modified Caged siRNAs for Photoregulating Exogenous and Endogenous Gene Expression
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-03-07 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00080
Jiali Yang 1 , Changmai Chen 1 , Xinjing Tang 1
Affiliation  

siRNA has been widely applied in research and drug development due to its sequence-specific gene silencing ability. However, how to spatiotemporally control its function is still one of its challenges. Light, a fast and noninvasive trigger, is a promising tool for spatiotemporal control of gene expression. Here, we designed and synthesized a new series of caged siRNAs modified with single cholesterol at the 5′ terminal of antisense strand RNA through a photolabile linker (Chol-PL-siRNAs). We demonstrated that these caged siRNAs were successfully used to photochemically regulate both exogenous (firefly luciferase and gfp) and endogenous gene expression (mitotic kinesin-5, Eg5) in cells.

中文翻译:

胆固醇修饰的笼状siRNA,用于光调节外源和内源基因的表达

siRNA具有序列特异性基因沉默功能,因此已广泛用于研究和药物开发。然而,如何时空控制其功能仍然是其挑战之一。光是一种快速且无创的触发手段,是用于时空控制基因表达的有前途的工具。在这里,我们设计并合成了一系列新的笼状siRNA,它们通过光不稳定连接子(Chol-PL-siRNA)在反义链RNA的5'末端被单个胆固醇修饰。我们证明,这些笼中的siRNA已成功用于光化学调节细胞中的外源性(萤火虫荧光素酶gfp)和内源性基因表达(有丝分裂驱动蛋白5,Eg5)。
更新日期:2018-03-07
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