The actinobacterium, strain M26^T, was isolated from garden soil that was pre-treated with microwave radiation. The soil sample was collected in Roodepoort, Gauteng Province, South Africa as part of an antibiotic-screening programme. The isolate produced branched vegetative mycelium with sporangiophores bearing small sporangia ranging from 3 to 6 μm in diameter. Rapid genus identification revealed that the isolate belongs to the genus Streptosporangium. To confirm this result, the strain was subjected to polyphasic taxonomic characterisation. Chemotaxonomic characteristics were as follows: meso-DAP in the peptidoglycan, the whole-cell hydrolysate yielded madurose, predominant menaquinones were MK9 (21%), MK9(H₂) (40%), MK9(H₄) (31%) and MK9(H₆) (3%); the polar lipid profile included an aminolipid, phosphoglycolipids, phosphatidylethanolamine, and phosphatidylmonomethylethanolamine. In addition, the fatty acid profile showed the presence of C_16:0 (12.8%), C_17:1ω8c (14.2%), and 10-methyl-C_17:0 (15.8%). Furthermore, 16S rRNA gene sequence phylogenetic analysis showed that the strain is closely related to members of the genus Streptosporangium, which supports its classification within the family Streptosporangiaceae. Strain M26^T exhibited antibiosis against a range of pathogenic bacteria, including, but not limited to Acinetobacter baumannii ATCC 19606^T, Enterobacter cloacae subsp. cloacae ATCC BAA-1143, Enterococcus faecalis ATCC 51299 (vancomycin resistant), Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19111, Mycobacterium tuberculosis H37Rv^T, Pseudomonas aeruginosa ATCC 27853, Salmonella enterica subsp. arizonae ATCC 13314^T, and the methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 (MRSA). The name Streptosporangium minutum is proposed with the type strain M26^T (=LMG 28850^T =NRRL B-65295^T).

中文翻译：

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细链霉菌 十一月，与暴露于微波辐射的花园土壤隔离。

的actinobacterium，应变M26 ^Ť，由园土，将其用微波辐射预处理隔离。作为抗生素筛选计划的一部分，在南非豪登省鲁德普特（Roodepoort）收集了土壤样品。该分离物产生分支的营养菌丝体，其孢子囊带有直径为3至6μm的小孢子囊。快速鉴定属表明该分离物属于链霉菌属。为了证实该结果，对该菌株进行了多相分类学表征。化学分类学特征如下：肽聚糖中的meso-DAP，全细胞水解产物产生的madurose，主要的甲基萘醌为MK9（21％），MK9（H ₂）（40％），MK9（H ₄）（31％）和MK9（H ₆）（3％）; 极性脂质分布包括氨基脂质，磷酸糖脂，磷脂酰乙醇胺和磷脂酰单甲基乙醇胺。另外，脂肪酸谱显示出存在C _16：0（12.8％），C _17： 1ω8c（14.2％）和10-甲基-C _17：0（15.8％）。此外，对16S rRNA基因序列的系统发育分析表明，该菌株与链霉菌属（Streptosporangium）属成员密切相关，这支持其在链霉菌科中的分类。M26 ^T菌株对多种病原菌具有抗生能力，包括但不限于鲍曼不动杆菌ATCC 19606 ^T，阴沟肠杆菌亚种。泄殖腔ATCC BAA-1143，粪肠球菌ATCC 51299（耐万古霉素），大肠杆菌ATCC 25922，单核细胞增生李斯特菌ATCC 19111，结核分枝杆菌H37Rv ^T，铜绿假单胞菌ATCC 27853，肠沙门氏菌亚种。亚利桑那州ATCC 13314 ^T和耐甲氧西林的金黄色葡萄球菌亚种。金黄色葡萄球菌ATCC 33591（MRSA）。提出了名称为Streptosporangium minutum的名称，名称为M26 ^T型菌株（= LMG 28850 ^T = NRRL B-65295 ^T）。