Cytotoxicity and genotoxicity of sodium acetate (SA), sodium diacetate (SDA), and potassium sorbate (PS) was tested on Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was investigated by MTT assay and flow cytometry analysis, while genotoxicity was evaluated using DNA fragmentation and DAPI staining assays. The growth of treated HUVECs with various concentrations of SA, SDA and PS decreased in a dose-and time-dependent manner. The IC50 of 487.71, 485.82 and 659.96 µM after 24 h and IC50 of 232.05, 190.19 and 123.95 µM after 48 h of treatment were attained for SA, SDA and PS, respectively. Flow cytometry analysis showed that early and late apoptosis percentage in treated cells was not considerable. Also neither considerable DNA fragmentation nor DNA smear was observed using DAPI staining and DNA ladder assays. Overall, it can be concluded that the aforementioned food additives can be used as safe additives at low concentration in food industry.

在人脐静脉内皮细胞（HUVEC）上测试了乙酸钠（SA），双乙酸钠（SDA）和山梨酸钾（PS）的细胞毒性和遗传毒性。通过MTT测定和流式细胞术分析研究细胞毒性，而使用DNA片段化和DAPI染色测定评价遗传毒性。不同浓度的SA，SDA和PS处理的HUVEC的生长呈剂量和时间依赖性降低。SA，SDA和PS在24小时后的IC50分别为487.71、485.82和659.96 µM，在48小时后的IC50分别为232.05、190.19和123.95 µM。流式细胞仪分析表明，在处理过的细胞中，早期和晚期凋亡百分数不大。同样，使用DAPI染色和DNA阶梯分析也未观察到明显的DNA片段化或DNA涂片。全面的，