In this work, a convenient and sensitive method for the determination of triazoles in environmental waters was developed by on-line combining in-tube solid phase microextraction (IT-SPME) and high performance liquid chromatography with diode array detector (HPLC-DAD). To extract triazoles effectively, poly (4-vinyl pyridine-co-ethylene dimethacrylate) monolith was in-situ fabricated and utilized as the extraction phase of IT-SPME. A series of key extraction parameters including desorption solvent, sample volume, adsorption and desorption flow rate, pH value and ionic strength in sample matrix were optimized thoroughly. Under the most favorable conditions (volume of sample, 6.0 mL; adsorption flow rate, 0.2 mL/min; desorption solvent, 80.0 µL mixture of ACN/water (70/30, v/v); desorption flow rate, 50.0 µL/min; sample pH value, 8.0; ionic strength did not be adjusted), the developed monolith-based IT-SPME could extract target analytes effectively and expected analytical merits were achieved. The limits of detection (S/N = 3) and limits of quantification (S/N = 10) were in the ranges of 0.014–0.031 µg/L and 0.11–0.074 µg/L, respectively. Satisfactory method reproducibility was obtained by intra-day and inter-day precisions, with relative standard deviations (RSDs) lower than 10%. The optimized IT-SPME-HPLC-DAD method was then applied to detect triadimenol, triazolone and hexaconazole in water samples including lake, river and sewage waters. The spiked recoveries were 78.9–106% and the RSDs were in the range of 0.2–7.2%. The results well evidence that the proposed method is convenient, accurate, sensitive, practical and environmentally friendly for the determination of triazoles in environmental waters.

在这项工作中，通过在线结合管内固相微萃取（IT-SPME）和高效液相色谱与二极管阵列检测器（HPLC-DAD），开发了一种简便而灵敏的测定环境水中三唑的方法。为了有效地提取三唑，原位制备了聚（4-乙烯基吡啶-共-乙烯-二甲基丙烯酸乙烯酯）整料制造并用作IT-SPME的提取阶段。彻底优化了解吸溶剂，样品量，吸附和解吸流速，pH值和样品基质中的离子强度等一系列关键提取参数。在最有利的条件下（样品量为6.0 mL；吸附流速为0.2 mL / min；解吸溶剂为80.0 µL乙腈/水的混合物（70/30，v / v）；解吸流速为50.0 µL / min ；样品的pH值为8.0；未调整离子强度），开发的基于整体的IT-SPME可以有效地萃取目标分析物，并达到了预期的分析优点。检测限（S / N = 3）和定量限（S / N = 10）分别在0.014–0.031 µg / L和0.11–0.074 µg / L的范围内。通过日内和日间精度获得令人满意的方法重现性，相对标准偏差（RSD）低于10％。然后将优化的IT-SPME-HPLC-DAD方法用于检测包括湖泊，河流和污水在内的水样中的三苯二酚，三唑酮和六康唑。加标回收率为78.9–106％，RSD在0.2–7.2％的范围内。结果充分证明了该方法简便，准确，灵敏，实用，环境友好，可用于环境水样中三唑类的测定。9–106％，相对标准偏差在0.2–7.2％的范围内。结果充分证明了该方法简便，准确，灵敏，实用，环境友好，可用于环境水样中三唑类的测定。9–106％，相对标准偏差在0.2–7.2％的范围内。结果充分证明了该方法简便，准确，灵敏，实用，环境友好，可用于环境水样中三唑类的测定。