In order to improve the osteogenic activity and mechanical strength of the guided bone regeneration (GBR) membrane for repairing bone defect, nano-hydroxyapatite/chitosan (nHA/CS) composite microspheres were prepared through in situ biomimetic method, then composite microspheres were incorporated into CS membrane. The morphologies and mechanical properties of the composite membranes were investigated through scanning electronic microscopy (SEM) and universal mechanical testing machine. The results show that the in situ biomimetic nHA/CS microspheres were embedded in CS membrane and were integrated tightly with CS matrix. The mechanical properties of GBR membranes containing in situ nHA/CS microspheres is significantly higher than that of membranes containing pure CS microspheres and blending nHA/CS microspheres. Its elongation rate at break reaches 5.61 ± 0.95%. The elastic modulus and strength of the GBR membranes can reach 766.27 ± 20.68 and 43.32 ± 0.95 MPa, respectively. Further, The work-of-fracture of the membranes with in situ microspheres approaches 2.71 ± 0.25 J/m², which is about 3 times of the pure CS membrane. The cell culture results display that the GBR membranes containing in situ biomimetic nHA/CS microspheres exhibit good cytocompatibility.

为了提高引导骨再生（GBR）膜修复骨缺损的成骨活性和机械强度，通过原位仿生法制备了纳米羟基磷灰石/壳聚糖（nHA / CS）复合微球，然后将复合微球掺入CS膜。通过扫描电子显微镜（SEM）和通用力学试验机研究了复合膜的形貌和力学性能。结果表明，原位仿生nHA / CS微球包埋在CS膜中，并与CS基质紧密结合。原位含GBR膜的力学性能nHA / CS微球明显高于包含纯CS微球和nHA / CS混合微球的膜。断裂伸长率达到5.61±0.95％。GBR膜的弹性模量和强度分别可以达到766.27±20.68和43.32±0.95 MPa。此外，具有原位微球的膜的断裂功接近2.71±0.25 J / m ²，约为纯CS膜的3倍。细胞培养结果表明，含有原位仿生nHA / CS微球的GBR膜具有良好的细胞相容性。