AbstractThe authors report on a loop-mediated isothermal amplification (LAMP) scheme that uses antarctic thermally sensitive uracil-DNA-glycosylase (AUDG) for simultaneous detection of nucleic acids and elimination of carryover contamination. It was applied in a lateral flow assay (LFA) format. The assay has attractive features in that it does not require the use of labeled primers or probes, and can eliminate false-positive results generated by unwanted hybridization between two labeled primers or between a labeled primer and probe. LAMP amplification and AUDG digestion are conducted in a single pot, and the application of a closed-tube reaction prevents false-positives due to carryover contamination. The method was applied to the detection of the human pathogen Streptococcus pneumoniaein in pure cultures and spiked blood samples. This LFA can detect S. pneumoniae in pure cultures with a 25 fg.μL−1 detection limit and in spiked blood samples with a 470 cfu.mL−1 detection limit. Conceivably, this assay can be applied to the detection of various other targets if the specific LAMP primers are available. Graphical abstractᅟ

中文翻译：

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在侧流生物传感器中使用环介导等温扩增和南极热敏尿嘧啶-DNA-糖基化酶检测核酸并消除残留污染：在肺炎链球菌检测中的应用

摘要作者报告了环介导等温扩增 (LAMP) 方案，该方案使用南极热敏尿嘧啶-DNA-糖基化酶 (AUDG) 同时检测核酸并消除残留污染。它被应用于横向流动分析 (LFA) 格式。该测定具有吸引人的特点，因为它不需要使用标记引物或探针，并且可以消除由两个标记引物之间或标记引物和探针之间不需要的杂交产生的假阳性结果。LAMP 扩增和 AUDG 消化在一个锅中进行，闭管反应的应用可防止由于残留污染而导致的假阳性。该方法用于检测纯培养物和加标血样中的人类病原体肺炎链球菌。该 LFA 可以检测纯培养物中的肺炎链球菌，检测限为 25 fg.μL-1，加标血样中的检测限为 470 cfu.mL-1。可以想象，如果有特定的 LAMP 引物，这种检测可以应用于检测各种其他目标。图形摘要ᅟ