Collagen export from the endoplasmic reticulum (ER) requires TANGO1, COPII coats, and retrograde fusion of ERGIC membranes. How do these components come together to produce a transport carrier commensurate with the bulky cargo collagen? TANGO1 is known to form a ring that corrals COPII coats, and we show here how this ring or fence is assembled. Our data reveal that a TANGO1 ring is organized by its radial interaction with COPII, and lateral interactions with cTAGE5, TANGO1-short or itself. Of particular interest is the finding that TANGO1 recruits ERGIC membranes for collagen export via the NRZ (NBAS/RINT1/ZW10) tether complex. Therefore, TANGO1 couples retrograde membrane flow to anterograde cargo transport. Without the NRZ complex, the TANGO1 ring does not assemble, suggesting its role in nucleating or stabilising this process. Thus, coordinated capture of COPII coats, cTAGE5, TANGO1-short, and tethers by TANGO1 assembles a collagen export machine at the ER.

中文翻译：

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TANGO1 通过招募和空间组织 COPII、系绳和膜来构建胶原蛋白输出机器

内质网 (ER) 的胶原蛋白输出需要 TANGO1、COPII 涂层和 ERGIC 膜的逆行融合。这些成分如何组合在一起以产生与庞大的货物胶原蛋白相称的运输载体？众所周知，TANGO1 会形成一个环，将 COPII 的外套围起来，我们在这里展示了这个环或围栏是如何组装的。我们的数据显示 TANGO1 环是通过其与 COPII 的径向相互作用以及与 cTAGE5、TANGO1-short 或自身的横向相互作用来组织的。特别令人感兴趣的是发现 TANGO1 通过 NRZ (NBAS/RINT1/ZW10) 系绳复合物招募 ERGIC 膜用于胶原蛋白输出。因此，TANGO1 将逆行膜流与顺行货物运输相结合。没有 NRZ 复合物，TANGO1 环不会组装，表明它在成核或稳定这个过程中的作用。因此，