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Fabrication of Various Structures of Nanostraw Arrays and Their Applications in Gene Delivery
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2018-03-07 , DOI: 10.1002/admi.201701535 Gen He 1 , Hui-Jiuan Chen 1 , Di Liu 2 , Yuping Feng 1 , Chengduan Yang 1 , Tian Hang 1 , Jiangming Wu 1 , Yuhong Cao 3 , Xi Xie 1, 3
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2018-03-07 , DOI: 10.1002/admi.201701535 Gen He 1 , Hui-Jiuan Chen 1 , Di Liu 2 , Yuping Feng 1 , Chengduan Yang 1 , Tian Hang 1 , Jiangming Wu 1 , Yuhong Cao 3 , Xi Xie 1, 3
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Nanowires have a wide variety of biomedical applications, including drug delivery, cellular manipulation, cellular interfacing, and biosensing. Specifically, hollow nanowire arrays, or “nanostraws,” are recently developed to penetrate cell membranes and to provide temporal and dose controlled delivery of biomolecules into the cells. The fabrication of nanostraw arrays uses nanoporous membranes as starting templates. However, nanoporous membranes with custom‐designed diameters and densities are often expensive or commercially unavailable. As a result, nanostraws reported in literature consist of a limited number of structures with restricted applications. In this work, a simple approach of preparing nanostraws with differing structures, including various diameters, lengths, and densities, is developed. Furthermore, the application of nanostraws in gene transfection is assessed. To fabricate the nanostraw arrays, O2 plasma etching is applied to the template membrane with a desirable pore density to produce nanopores with matching sizes. Then, a series of nanostraws with controllable diameters, densities, and lengths is consistently fabricated. Finally, the nanostraws are integrated with a microfluidic device to penetrate the cell membrane or coupled with external techniques to achieve DNA transfection. This work demonstrates a simple yet versatile approach to fabricate a variety of hollow nanowires for broad biomedical device applications.
中文翻译：
纳米吸管阵列的各种结构的制备及其在基因传递中的应用
纳米线具有广泛的生物医学应用，包括药物输送，细胞操纵，细胞接口和生物传感。具体而言，近来开发了中空纳米线阵列或“ nanostraws”以穿透细胞膜，并提供时间和剂量控制的生物分子向细胞内的递送。纳米秸秆阵列的制造使用纳米多孔膜作为起始模板。但是，具有定制设计的直径和密度的纳米多孔膜通常很昂贵或在商业上不可用。结果，文献报道的纳米吸管由有限数量的具有受限应用的结构组成。在这项工作中，开发了一种简单的方法来制备具有不同结构（包括各种直径，长度和密度）的纳米吸管。此外，评估了纳米吸管在基因转染中的应用。为了制造纳米吸管阵列，O2将等离子蚀刻以所需的孔密度施加到模板膜上，以产生具有匹配尺寸的纳米孔。然后，始终如一地制造一系列具有可控制的直径，密度和长度的纳米吸管。最后，将纳米吸管与微流控设备集成在一起以穿透细胞膜，或者与外部技术结合以实现DNA转染。这项工作展示了一种简单而通用的方法，可以为广泛的生物医学设备应用制造各种空心纳米线。
更新日期：2018-03-07
中文翻译：
纳米吸管阵列的各种结构的制备及其在基因传递中的应用
纳米线具有广泛的生物医学应用，包括药物输送，细胞操纵，细胞接口和生物传感。具体而言，近来开发了中空纳米线阵列或“ nanostraws”以穿透细胞膜，并提供时间和剂量控制的生物分子向细胞内的递送。纳米秸秆阵列的制造使用纳米多孔膜作为起始模板。但是，具有定制设计的直径和密度的纳米多孔膜通常很昂贵或在商业上不可用。结果，文献报道的纳米吸管由有限数量的具有受限应用的结构组成。在这项工作中，开发了一种简单的方法来制备具有不同结构（包括各种直径，长度和密度）的纳米吸管。此外，评估了纳米吸管在基因转染中的应用。为了制造纳米吸管阵列，O2将等离子蚀刻以所需的孔密度施加到模板膜上，以产生具有匹配尺寸的纳米孔。然后，始终如一地制造一系列具有可控制的直径，密度和长度的纳米吸管。最后，将纳米吸管与微流控设备集成在一起以穿透细胞膜，或者与外部技术结合以实现DNA转染。这项工作展示了一种简单而通用的方法，可以为广泛的生物医学设备应用制造各种空心纳米线。
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