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Neuropeptidome of the Hypothalamus and Pituitary Gland of Indicine × Taurine Heifers: Evidence of Differential Neuropeptide Processing in the Pituitary Gland before and after Puberty
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-04-06 00:00:00 , DOI: 10.1021/acs.jproteome.7b00875 Kasey L DeAtley 1 , Michelle L Colgrave 2 , Angela Cánovas 3 , Gene Wijffels 2 , Ryan L Ashley 1 , Gail A Silver 1 , Gonzalo Rincon 4 , Juan F Medrano 5 , Alma Islas-Trejo 5 , Marina R S Fortes 6, 7 , Antonio Reverter 2 , Laercio Porto-Neto 2 , Sigrid A Lehnert 2 , Milton G Thomas 8
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-04-06 00:00:00 , DOI: 10.1021/acs.jproteome.7b00875 Kasey L DeAtley 1 , Michelle L Colgrave 2 , Angela Cánovas 3 , Gene Wijffels 2 , Ryan L Ashley 1 , Gail A Silver 1 , Gonzalo Rincon 4 , Juan F Medrano 5 , Alma Islas-Trejo 5 , Marina R S Fortes 6, 7 , Antonio Reverter 2 , Laercio Porto-Neto 2 , Sigrid A Lehnert 2 , Milton G Thomas 8
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Puberty in cattle is regulated by an endocrine axis, which includes a complex milieu of neuropeptides in the hypothalamus and pituitary gland. The neuropeptidome of hypothalamic–pituitary gland tissue of pre- (PRE) and postpubertal (POST) Bos indicus-influenced heifers was characterized, followed by quantitative analysis of 51 fertility-related neuropeptides in these tissues. Comparison of peptide abundances with gene expression levels allowed assessment of post-transcriptional peptide processing. On the basis of classical cleavage, 124 mature neuropeptides from 35 precursor proteins were detected in hypothalamus and pituitary gland tissues of three PRE and three POST Brangus heifers. An additional 19 peptides (cerebellins, PEN peptides) previously reported as neuropeptides that did not follow classical cleavage were also identified. In the pre-pubertal hypothalamus, a greater diversity of neuropeptides (25.8%) was identified relative to post-pubertal heifers, while in the pituitary gland, 38.6% more neuropeptides were detected in the post-pubertal heifers. Neuro-tissues of PRE and POST heifers revealed abundance differences (p < 0.05) in peptides from protein precursors involved in packaging and processing (e.g., the granin family and ProSAAS) or neuron stimulation (PENK, CART, POMC, cerebellins). On their own, the transcriptome data of the precursor genes could not predict the neuropeptide profile in the exact same tissues in several cases. This provides further evidence of the importance of differential processing of the neuropeptide precursors in the pituitary before and after puberty.
中文翻译：
印度×牛磺酸小母牛下丘脑和脑垂体的神经肽组：青春期前后脑垂体神经肽加工差异的证据
牛的青春期由内分泌轴调节，内分泌轴包括下丘脑和垂体中复杂的神经肽环境。对受Bos indicus影响的小母牛的下丘脑 - 垂体腺组织的神经肽组进行了表征，然后对这些组织中的 51 种与生育相关的神经肽进行了定量分析。肽丰度与基因表达水平的比较允许评估转录后肽加工。在经典裂解的基础上，在三头 PRE 和三头 POST Brangus 小母牛的下丘脑和垂体组织中检测到来自 35 种前体蛋白的 124 种成熟神经肽。另外还鉴定了 19 种先前报道为不遵循经典裂解的神经肽的肽（小脑蛋白、PEN 肽）。在青春期前的下丘脑中，相对于青春期后的小母牛，发现了更多的神经肽多样性（25.8%），而在垂体中，在青春期后的小母牛中检测到了38.6%的神经肽。 PRE 和 POST 小母牛的神经组织揭示了来自参与包装和加工（例如，granin 家族和 ProSAAS）或神经元刺激（PENK、CART、POMC、小脑蛋白）的蛋白质前体的肽的丰度差异（ p < 0.05）。在某些情况下，前体基因的转录组数据本身无法预测完全相同组织中的神经肽谱。这进一步证明了青春期前后垂体中神经肽前体的差异加工的重要性。
更新日期：2018-04-07
中文翻译：
印度×牛磺酸小母牛下丘脑和脑垂体的神经肽组：青春期前后脑垂体神经肽加工差异的证据
牛的青春期由内分泌轴调节，内分泌轴包括下丘脑和垂体中复杂的神经肽环境。对受Bos indicus影响的小母牛的下丘脑 - 垂体腺组织的神经肽组进行了表征，然后对这些组织中的 51 种与生育相关的神经肽进行了定量分析。肽丰度与基因表达水平的比较允许评估转录后肽加工。在经典裂解的基础上，在三头 PRE 和三头 POST Brangus 小母牛的下丘脑和垂体组织中检测到来自 35 种前体蛋白的 124 种成熟神经肽。另外还鉴定了 19 种先前报道为不遵循经典裂解的神经肽的肽（小脑蛋白、PEN 肽）。在青春期前的下丘脑中，相对于青春期后的小母牛，发现了更多的神经肽多样性（25.8%），而在垂体中，在青春期后的小母牛中检测到了38.6%的神经肽。 PRE 和 POST 小母牛的神经组织揭示了来自参与包装和加工（例如，granin 家族和 ProSAAS）或神经元刺激（PENK、CART、POMC、小脑蛋白）的蛋白质前体的肽的丰度差异（ p < 0.05）。在某些情况下，前体基因的转录组数据本身无法预测完全相同组织中的神经肽谱。这进一步证明了青春期前后垂体中神经肽前体的差异加工的重要性。
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