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Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-06 00:00:00 , DOI: 10.1021/acs.analchem.7b04419 Huiying Xu 1 , Chong Liao 1 , Yujie Liu 2 , Bang-Ce Ye 1 , Baohong Liu 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-06 00:00:00 , DOI: 10.1021/acs.analchem.7b04419 Huiying Xu 1 , Chong Liao 1 , Yujie Liu 2 , Bang-Ce Ye 1 , Baohong Liu 2
Affiliation
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Nitric oxide (NO) is a transcellular messenger involved in many physiological and pathological processes, but the real-time detection of NO in biological systems is still challenging due to its rapid diffusion, low concentration, and short half-life. A novel electrochemical sensing platform based on iron phthalocyanine (FePc) functionalized nitrogen-doped graphene (N-G) nanocomposites was constructed to achieve in situ monitoring of NO released from living cells on the sensing layer. By taking advantage of the synergetic effect of N-G and FePc nanocomposites, the N-G/FePc sensor displays excellent electrocatalytic activity toward NO with a high sensitivity of 0.21 μA μM–1 cm–2 and a low detection limit of 180 nmol L–1. The following layer-by-layer assembly of poly-l-lysine (PLL) and Nafion further improved the capacity of resisting disturbance as well as the biocompatibility of the sensing interface. The flexible design of the ITO substrate based electrode provides a more controlled cellular biosensing system which could capture molecular signals immediately after NO released from human umbilical vein endothelial cells (HUVECs). The exhibited additional features of high sensitivity, rapid response, and ease of operation implies that the proposed N-G/FePc/Nafion/PLL ITO biosensor is a promising powerful platform in various complex biological systems.
中文翻译：
酞菁铁修饰的氮掺杂石墨烯生物传感平台用于实时检测从活细胞释放的一氧化氮
一氧化氮（NO）是涉及许多生理和病理过程的跨细胞信使，但是由于其快速扩散，低浓度和短半衰期，因此在生物系统中实时检测NO仍然具有挑战性。构建了一种基于铁酞菁（FePc）功能化的氮掺杂石墨烯（NG）纳米复合材料的新型电化学传感平台，以实现对传感层上活细胞释放的NO的原位监测。通过利用NG和FePc纳米复合材料的协同作用，NG / FePc传感器对NO表现出出色的电催化活性，具有0.21μAμM –1 cm –2的高灵敏度和180 nmol L –1的低检测限。以下层-层组装的聚升-赖氨酸（PLL）和Nafion进一步提高抗干扰能力，以及所述感测接口的生物相容性。基于ITO基板的电极的灵活设计提供了一个更加可控的细胞生物传感系统，该系统可以在NO从人脐静脉内皮细胞（HUVEC）释放后立即捕获分子信号。展示的高灵敏度，快速响应和易于操作的其他特征意味着，所提出的NG / FePc / Nafion / PLL ITO生物传感器是各种复杂生物系统中有希望的强大平台。
更新日期：2018-03-06
中文翻译：
酞菁铁修饰的氮掺杂石墨烯生物传感平台用于实时检测从活细胞释放的一氧化氮
一氧化氮（NO）是涉及许多生理和病理过程的跨细胞信使，但是由于其快速扩散，低浓度和短半衰期，因此在生物系统中实时检测NO仍然具有挑战性。构建了一种基于铁酞菁（FePc）功能化的氮掺杂石墨烯（NG）纳米复合材料的新型电化学传感平台，以实现对传感层上活细胞释放的NO的原位监测。通过利用NG和FePc纳米复合材料的协同作用，NG / FePc传感器对NO表现出出色的电催化活性，具有0.21μAμM –1 cm –2的高灵敏度和180 nmol L –1的低检测限。以下层-层组装的聚升-赖氨酸（PLL）和Nafion进一步提高抗干扰能力，以及所述感测接口的生物相容性。基于ITO基板的电极的灵活设计提供了一个更加可控的细胞生物传感系统，该系统可以在NO从人脐静脉内皮细胞（HUVEC）释放后立即捕获分子信号。展示的高灵敏度，快速响应和易于操作的其他特征意味着，所提出的NG / FePc / Nafion / PLL ITO生物传感器是各种复杂生物系统中有希望的强大平台。
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