Bimodal Electrochemiluminescence of G-CNQDs in the Presence of Double Coreactants for Ascorbic Acid Detection,Analytical Chemistry

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Bimodal Electrochemiluminescence of G-CNQDs in the Presence of Double Coreactants for Ascorbic Acid Detection
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-06 00:00:00 , DOI: 10.1021/acs.analchem.8b00517
Huan Wang _{1,

2} , Guiqiang Pu ₁ , Samrat Devaramani ₁ , Yanfeng Wang ₁ , Zhaofan Yang ₁ , Linfang Li ₁ , Xiaofang Ma ₁ , Xiaoquan Lu _{1,

3}

Affiliation

How to improve the accuracy of target detection substance in low-content and complex of real sample, which is still a major challenge in the analysis field. There is no doubt that the internal standard method is the best choice in the analysis methods. The internal standard method of ECL strategy can furnish more accurate detection results in the changeable complex environment, and it can dispel the primary vaguest interference in the system through the self-calibration of two emission spectra. Herein, we effectually explored a strong and stable bimodal ECL system based on graphitic carbon nitride quantum dots (g-CNQDs) as single luminophore in the presence of double coreactants potassium persulfate (K₂S₂O₈) and tetrabutylammonium bromide (TBAB) under the optimized conditions. ECL-1 at 2.82 V and ECL-2 at 1.73 V were observed when the potential was scanned between −3 and 3 V at the scan rate of 0.2 V·s^–1. The ECL-1 was responding to the analyte, that is, ascorbic acid (AA) and the ECL-2 was not for a certain concentration of AA; hence, the developed bimodal ECL system was used as internal standard method for quantitative AA in human serum due to the different sensitivity of the double-peak ECL signals to the target analytes. The linear relationships were obtained based on the ln I (ECL-1/ECL-2) against the concentration of AA in the concentration range of 3.5 to 330 nM, with a detection limit of 110 pM (S/N = 3).

中文翻译：

存在双抗坏血酸检测的双共反应物存在下G-CNQDs的双峰电化学发光

如何提高目标样品中低含量，复杂样品中目标检测物质的准确性，仍然是分析领域的主要挑战。毫无疑问，内标法是分析方法中的最佳选择。ECL策略的内标方法可以在多变的复杂环境中提供更准确的检测结果，并且可以通过两个发射光谱的自校准消除系统中最主要的模糊干扰。在此，我们有效地探索了在双共反应物过硫酸钾（K ₂ S ₂ O _8）存在下，基于石墨化碳氮化物量子点（g-CNQDs）作为单个发光体的强大而稳定的双峰ECL系统。）和四丁基溴化铵（TBAB）在最佳条件下进行。当以0.2 V·s ^–1的扫描速率在-3至3 V之间扫描电势时，观察到2.82 V的ECL-1和1.73 V的ECL-2 。ECL-1对分析物有反应，即抗坏血酸（AA），而ECL-2对一定浓度的AA没有反应。因此，由于双峰ECL信号对目标分析物的敏感性不同，开发的双峰ECL系统被用作人血清中AA定量的内标方法。根据ln I（ECL-1 / ECL-2）对AA浓度在3.5至330 nM的浓度范围内获得线性关系，检出限为110 pM（S / N = 3）。

更新日期：2018-03-06

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