The chemistry, structure and morphology of the implant surface have a great influence on the integration of an implant material with bone tissue. In this work, we applied Combinatorial Matrix-Assisted Pulsed Laser Evaporation (C-MAPLE) to deposit gradient thin films with variable compositions of Sr-substituted hydroxyapatite (SrHA) and Zn-substituted β-tricalcium phosphate (ZnTCP) on Titanium substrates. Five samples with different SrHA/ZnTCP composition ratios were fabricated by a single step laser procedure. SrHA was synthesized in aqueous medium, whereas ZnTCP was obtained by reaction at high temperature. Both powders were separately suspended in deionized water, frozen at liquid nitrogen temperature and used as targets for C-MAPLE experiments, which proceed via simultaneous laser vaporization of two distinct material targets. X-ray diffraction, scanning electron microscopy and energy dispersive X-ray spectroscopy analyses confirmed that the coatings contain the same crystalline phases as the as-prepared powder samples, with a homogeneous distribution of the two phosphates along deposited thin films. Human osteoclast precursor 2T-110 and human osteoblast-like cells MG63 were co-cultured on the coatings. The results indicate that osteoblast viability and production of osteocalcin were promoted by the presence of ZnTCP. On the other hand, SrHA inhibited osteoclastogenesis and osteoclast differentiation, as demonstrated by the observed increase of the osteoprotegerin/RANKL ratio and decrease of the number of TRAP-positive multinucleated cells when increasing SrHA amount in the coatings. The results indicate that the possibility to tailor the composition of the coatings provides materials able to modulate bone growth and bone resorption.

植入物表面的化学，结构和形态对植入物材料与骨组织的整合有很大影响。在这项工作中，我们应用了组合矩阵辅助脉冲激光蒸发（C-MAPLE）在钛基板上沉积具有可变组成的Sr取代羟基磷灰石（SrHA）和Zn取代β-磷酸三钙（ZnTCP）的梯度薄膜。通过一步激光法制备了五个具有不同SrHA / ZnTCP组成比的样品。SrHA是在水性介质中合成的，而ZnTCP是通过高温反应获得的。将两种粉末分别悬浮在去离子水中，在液氮温度下冷冻，并用作C-MAPLE实验的靶标，该实验通过同时激光蒸发两个不同的材料靶标进行。X射线衍射，扫描电子显微镜和能量色散X射线光谱分析证实，涂层包含与制备的粉末样品相同的晶相，两种磷酸盐沿沉积的薄膜均匀分布。将人破骨细胞前体2T-110和人成骨细胞样细胞MG63共培养在涂层上。结果表明，ZnTCP的存在促进了成骨细胞的活力和骨钙素的产生。另一方面，SrHA抑制破骨细胞生成和破骨细胞分化，这通过观察到的增加涂层中SrHA量的骨保护素/ RANKL比值的增加和TRAP阳性多核细胞数量的减少来证明。