A novel electrochemical immunosensor based on carbon paste electrode (CPE) composed of ionic liquid (IL) and graphite was constructed. It demonstrated good efficiency for quick (each test in 30 s) determination of thyroid stimulating hormone (TSH). Electrode surface was modified by gold nanoparticles in order to immobilize of the thyroid stimulating hormone antibody (anti-TSH) on the CPE. The immunoassay structure was established by sandwiching the antigen (TSH) between the thyroid stimulating hormone antibody on the CPE surface modified with gold nanoparticles and the secondary antibody, polyclonal anti-human-TSH labeled with horseradish peroxidase (HRP-labeled anti-TSH). The signal of differential pulse voltammetry (DPV) was used as a basis for the determination of TSH concentration. This signal is generated by the reaction between O-aminophenol (OAP) and H₂O₂ catalyzed by HRP. The proposed immunosensor is able to measure the concentration of TSH in a linear range between 0.2 and 90.0 ng/mL with a detection limit 0.1 ± 0.02 ng/mL. In addition, high sensitivity and acceptable stability were achieved by this immunosensor which is promising in the clinical assay of TSH.

基于离子液体（IL）和石墨的碳糊电极（CPE）构建了一种新型的电化学免疫传感器。它证明了快速（每次测试30秒）测定甲状腺刺激激素（TSH）的良好效率。电极表面用金纳米颗粒修饰，以将甲状腺刺激激素抗体（抗TSH）固定在CPE上。通过将抗原（TSH）夹在用金纳米颗粒修饰的CPE表面的甲状腺刺激激素抗体与二抗（用辣根过氧化物酶标记的多克隆抗人TSH（HRP标记的抗TSH））之间来建立免疫测定结构。差分脉冲伏安法（DPV）的信号用作确定TSH浓度的基础。HRP催化₂ O ₂。所提出的免疫传感器能够在0.2到90.0 ng / mL的线性范围内测量TSH的浓度，检测限为0.1±0.02 ng / mL。另外，通过该免疫传感器实现了高灵敏度和可接受的稳定性，这在TSH的临床测定中是有希望的。