Natural Product Diversification Mediated by Alternative Transcriptional Starting,Angewandte Chemie International Edition

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Natural Product Diversification Mediated by Alternative Transcriptional Starting
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-04-14 , DOI: 10.1002/anie.201713199
Nicholas J. Tobias ₁ , Annabell Linck ₁ , Helge B. Bode _{1,

2}

Affiliation

Photorhabdus luminescens dedicates a significant proportion of its genome to the production of natural products. These products and the structural variation in their derivatives may occur by a number of well‐described mechanisms, such as module skipping or precursor promiscuity. Cappable‐seq was used to identify transcriptional start sites of many of the gene clusters present in P. luminescens TTO1. We discovered that variations associated with the non‐ribosomal peptide synthetase Kol, which is responsible for kolossin A production, possessed a number of internal transcripts that lead to synthesis of the smaller kolossin derivatives kolossin B and C. The data here support a new mechanism of natural product biosynthetic variation whereby mRNA may code for shorter NRPS enzymes in addition to full‐length proteins, resulting in the production of smaller peptide derivatives.

中文翻译：

替代性转录起始介导的天然产物多样化

发光的Photorhabdus luminescens将其基因组的很大一部分专用于天然产物的生产。这些产品及其衍生产品的结构变化可能是由许多描述良好的机制引起的，例如模块跳过或前体混杂。Cappable-seq用于鉴定发光假单胞菌中许多基因簇的转录起始位点TTO1。我们发现，与负责核糖蛋白A产生的非核糖体肽合成酶Kol相关的变异具有许多内部转录本，这些转录本可导致合成较小的核糖蛋白衍生物Kolossin B和C。此处的数据支持了一种新的机制天然产物的生物合成变异，除全长蛋白质外，mRNA还可编码较短的NRPS酶，从而产生较小的肽衍生物。

更新日期：2018-04-14

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