Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments,Angewandte Chemie International Edition

当前位置： X-MOL 学术 › Angew. Chem. Int. Ed. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-04-25 , DOI: 10.1002/anie.201800220
Sam Asami ₁ , Wolfgang Kallies ₂ , Johannes C. Günther ₁ , Maria Stavropoulou ₁ , Steffen J. Glaser ₂ , Michael Sattler _{1,

3}

Affiliation

NMR spectroscopy at ultra‐high magnetic fields requires improved radiofrequency (rf) pulses to cover the increased spectral bandwidth. Optimized 90° pulse pairs were introduced as Ramsey‐type cooperative (Ram‐COOP) pulses for biomolecular NMR applications. The Ram‐COOP element provides broadband excitation with enhanced sensitivity and reduced artifacts even at magnetic fields >1.0 GHz ¹H Larmor frequency (23 T). A pair of 30 μs Ram‐COOP pulses achieves an excitation bandwidth of 100 kHz with a maximum rf field of 20 kHz, more than three‐fold improved compared to excitation by rectangular pulses. Ram‐COOP pulses exhibit little offset‐dependent phase errors and are robust to rf inhomogeneity. The performance of the Ram‐COOP element is experimentally confirmed with heteronuclear multidimensional NMR experiments, applied to proteins and nucleic acids. Ram‐COOP provides broadband excitation at low rf field strength suitable for application at current magnetic fields and beyond 23 T.

中文翻译：

用于多维生物分子NMR实验的超短宽带协作脉冲

超高磁场下的NMR光谱法要求改进的射频（rf）脉冲以覆盖增加的光谱带宽。将优化的90°脉冲对作为Ramsey型协作（Ram-COOP）脉冲引入，用于生物分子NMR应用。Ram-COOP元件即使在大于1.0 GHz ¹ H拉莫尔频率（23 T）的磁场下也能提供宽带激励，具有更高的灵敏度并减少了伪影。一对30μs的Ram-COOP脉冲达到100 kHz的激发带宽，最大rf场为20 kHz，与矩形脉冲激发相比提高了三倍以上。Ram-COOP脉冲几乎没有偏移相关的相位误差，并且对rf具有鲁棒性不均匀性。Ram-COOP元件的性能已通过应用于蛋白质和核酸的异核多维NMR实验得到了实验证实。Ram‐COOP可在低rf场强下提供宽带激励，适用于当前磁场和超过23 T的磁场。

更新日期：2018-04-25

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11