Ultrafiltration liquid chromatography-mass spectrometry (ultrafiltration LC/MS) is introduced as an efficient method that can be applied to rapidly screen and identify ligands from the leaves of Ligustrum lucidum Ait. Using this method, we identified 13 compounds, including organic acids, flavonoids, and glycosides, as potent neuraminidase inhibitors. A continuous online method, employing pressurized liquid extraction followed by parallel centrifugal partition chromatography and preparative liquid chromatography PLE-(parallel-CPC/PLC), was developed for the efficient, scaled-up production of 12 compounds with high purities. The bioactivities of the separated compounds were assessed by an in vitro enzyme inhibition assay. The use of ultrafiltration LC/MS combined with PLE-(parallel-CPC/PLC), and an in vitro enzyme inhibition assay facilitated the efficient screening and isolation of neuraminidase inhibitors from complex samples, and could serve as an important platform for the large-scale production of functional ingredients.

超滤液相色谱-质谱法（超滤LC / MS）是一种有效的方法，可用于从女贞叶中快速筛选和鉴定配体。使用这种方法，我们确定了13种化合物（包括有机酸，类黄酮和糖苷）是有效的神经氨酸酶抑制剂。开发了一种连续在线方法，该方法采用加压液体萃取，然后进行平行离心分配色谱和制备液相色谱PLE-（parallel-CPC / PLC），以高效，规模化生产12种高纯度化合物。通过体外评估分离出的化合物的生物活性酶抑制试验。超滤LC / MS与PLE-（parallel-CPC / PLC）结合使用以及体外酶抑制试验有助于从复杂样品中有效筛选和分离神经氨酸酶抑制剂，并可以作为大样本的重要平台。规模生产功能性成分。