Genetically encoded fluorescent sensors enable cell-specific measurements of ions and small molecules in real time. Cyclic adenosine monophosphate (cAMP) is one of the most important signaling molecules in virtually all cell types and organisms. We describe cAMPr, a new single-wavelength cAMP sensor. We developed cAMPr in bacteria and embryonic stem cells and validated the sensor in mammalian neurons in vitro and in Drosophila circadian pacemaker neurons in intact brains. Comparison with other single-wavelength cAMP sensors showed that cAMPr improved the quantitative detection of cAMP abundance. In addition, cAMPr is compatible with both single-photon and two-photon imaging. This enabled us to use cAMPr together with the red fluorescent Ca²⁺ sensor RCaMP1h to simultaneously monitor Ca²⁺ and cAMP in Drosophila brains. Thus, cAMPr is a new and versatile genetically encoded cAMP sensor.

遗传编码的荧光传感器可以实时对离子和小分子进行细胞特异性测量。在几乎所有细胞类型和生物中，环磷酸一腺苷（cAMP）是最重要的信号分子之一。我们介绍了cAMPr，这是一种新型的单波长cAMP传感器。我们在细菌和胚胎干细胞中开发了cAMPr，并在体外和完整大脑中的果蝇昼夜节律起搏器神经元中验证了该传感器的功能。与其他单波长cAMP传感器的比较表明，cAMPr改善了cAMP丰度的定量检测。另外，cAMPr与单光子成像和双光子成像都兼容。这使我们能够将cAMPr与红色荧光Ca ²⁺传感器RCaMP1h一起使用，以同时监视Ca果蝇大脑中的²⁺和cAMP 。因此，cAMPr是一种新型的通用基因编码cAMP传感器。