AbstractAn ultrasensitive immunosensor for the direct detection of the illegally used livestock feed clebuterol (CLB) is described. It is based on the use of a glassy carbon electrode modified with an MoS2-AuPt nanocomposite and on biotin-streptavidin interaction. The use of MoS2-AuPt accelerates electron transfer, and this leads to a sharp increase in the electrochemical signal for the electrochemical probe hydrogen peroxide. Differential pulse voltammetry was used to record the current signal at a peak potential of −0.18 V (vs SCE). Under optimal conditions, the electrode has a linear response in the 10 pg·mL−1 to 100 ng·mL−1 CLB concentration range and a 6.9 pg·mL−1 detection limit (based on the 3σ criterium). This immunosensor is sensitive, highly specific and acceptably reproducible, and thus represents a valuable tool for the determination of CLB in pork. Graphical abstractSchematic of a voltammetric immunosensor for the determination of clenbuterol (CLB) based on the use of a nanocomposite prepared from molybdenum disulfide and a gold-platinum alloy (MoS2-AuPt), and making use of the biotin-streptavidin system.

中文翻译：

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基于使用 MoS2-AuPt 纳米复合材料的克仑特罗伏安免疫传感器

摘要描述了一种用于直接检测非法使用的牲畜饲料克利布特罗 (CLB) 的超灵敏免疫传感器。它基于使用 MoS2-AuPt 纳米复合材料修饰的玻璃碳电极和生物素-链霉亲和素相互作用。MoS2-AuPt 的使用加速了电子转移，这导致电化学探针过氧化氢的电化学信号急剧增加。差分脉冲伏安法用于记录峰值电位为 -0.18 V (vs SCE) 的电流信号。在最佳条件下，电极在 10 pg·mL-1 至 100 ng·mL-1 CLB 浓度范围内具有线性响应，检测限为 6.9 pg·mL-1（基于 3σ 标准）。这种免疫传感器灵敏、高度特异且重现性好，因此是测定猪肉中 CLB 的宝贵工具。图形摘要基于使用由二硫化钼和金铂合金 (MoS2-AuPt) 制备的纳米复合材料并利用生物素-链霉亲和素系统测定克仑特罗 (CLB) 的伏安免疫传感器示意图。