Nanoporous Anodic Alumina Surface Modification by Electrostatic, Covalent, and Immune Complexation Binding Investigated by Capillary Filling,ACS Applied Materials & Interfaces

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Nanoporous Anodic Alumina Surface Modification by Electrostatic, Covalent, and Immune Complexation Binding Investigated by Capillary Filling
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-03-06 00:00:00 , DOI: 10.1021/acsami.8b00572
Chris Eckstein ₁ , Laura K. Acosta ₁ , Laura Pol ₁ , Elisabet Xifré-Pérez ₁ , Josep Pallares ₁ , Josep Ferré-Borrull ₁ , Lluis F. Marsal ₁

Affiliation

The fluid imbibition-coupled laser interferometry (FICLI) technique has been applied to detect and quantify surface changes and pore dimension variations in nanoporous anodic alumina (NAA) structures. FICLI is a noninvasive optical technique that permits the determination of the NAA average pore radius with high accuracy. In this work, the technique is applied after each step of different surface modification paths of the NAA pores: (i) electrostatic immobilization of bovine serum albumin (BSA), (ii) covalent attachment of streptavidin via (3-aminipropyl)-triethoxysilane and glutaraldehyde grafting, and (iii) immune complexation. Results show that BSA attachment can be detected as a reduction in estimated radius from FICLI with high accuracy and reproducibility. In the case of the covalent attachment of streptavidin, FICLI is able to recognize a multilayer formation of the silane and the protein. For immune complexation, the technique is able to detect different antibody–antigen bindings and distinguish different dynamics among different immune species.

中文翻译：

通过毛细管填充研究静电，共价和免疫络合结合的纳米多孔阳极氧化铝表面改性。

流体吸收耦合激光干涉术（FICLI）技术已应用于检测和量化纳米多孔阳极氧化铝（NAA）结构中的表面变化和孔尺寸变化。FICLI是一种非侵入性光学技术，可以高精度确定NAA平均孔径。在这项工作中，该技术在NAA孔的不同表面修饰路径的每个步骤之后应用：（i）牛血清白蛋白（BSA）的静电固定，（ii）链霉亲和素通过（3-aminipropyl）-三乙氧基硅烷的共价连接和戊二醛接枝和（iii）免疫复合。结果表明，BSA附着可以检测为FICLI估计半径的减小，具有较高的准确性和可重复性。在链霉亲和素共价结合的情况下，FICLI能够识别硅烷和蛋白质的多层结构。对于免疫复合，该技术能够检测不同的抗体-抗原结合，并区分不同免疫物种之间的不同动态。

更新日期：2018-03-06

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