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Dynamical Magnetic Response of Iron Oxide Nanoparticles Inside Live Cells
ACS Nano ( IF 15.8 ) Pub Date : 2018-03-06 00:00:00 , DOI: 10.1021/acsnano.7b08995 David Cabrera 1, 2 , Annelies Coene 3 , Jonathan Leliaert 4 , Emilio J. Artés-Ibáñez 1 , Luc Dupré 3 , Neil D. Telling 2 , Francisco J. Teran 1, 5
ACS Nano ( IF 15.8 ) Pub Date : 2018-03-06 00:00:00 , DOI: 10.1021/acsnano.7b08995 David Cabrera 1, 2 , Annelies Coene 3 , Jonathan Leliaert 4 , Emilio J. Artés-Ibáñez 1 , Luc Dupré 3 , Neil D. Telling 2 , Francisco J. Teran 1, 5
Affiliation
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Magnetic nanoparticles exposed to alternating magnetic fields have shown a great potential acting as magnetic hyperthermia mediators for cancer treatment. However, a dramatic and unexplained reduction of the nanoparticle magnetic heating efficiency has been evidenced when nanoparticles are located inside cells or tissues. Recent studies suggest the enhancement of nanoparticle clustering and/or immobilization after interaction with cells as possible causes, although a quantitative description of the influence of biological matrices on the magnetic response of magnetic nanoparticles under AC magnetic fields is still lacking. Here, we studied the effect of cell internalization on the dynamical magnetic response of iron oxide nanoparticles (IONPs). AC magnetometry and magnetic susceptibility measurements of two magnetic core sizes (11 and 21 nm) underscored differences in the dynamical magnetic response following cell uptake with effects more pronounced for larger sizes. Two methodologies have been employed for experimentally determining the magnetic heat losses of magnetic nanoparticles inside live cells without risking their viability as well as the suitability of magnetic nanostructures for in vitro hyperthermia studies. Our experimental results—supported by theoretical calculations—reveal that the enhancement of intracellular IONP clustering mainly drives the cell internalization effects rather than intracellular IONP immobilization. Understanding the effects related to the nanoparticle transit into live cells on their magnetic response will allow the design of nanostructures containing magnetic nanoparticles whose dynamical magnetic response will remain invariable in any biological environments, allowing sustained and predictable in vivo heating efficiency.
中文翻译：
活细胞内氧化铁纳米粒子的动态磁响应
暴露在交变磁场中的磁性纳米粒子已显示出巨大的潜力，可作为磁高温疗法用于癌症治疗。然而，当纳米颗粒位于细胞或组织内部时，已经证明了纳米颗粒磁加热效率的显着和无法解释的降低。最近的研究表明，与细胞相互作用后，纳米颗粒的聚集和/或固定化的增强是可能的原因，尽管仍然缺乏关于在交流磁场下生物基质对磁性纳米颗粒磁响应的影响的定量描述。在这里，我们研究了细胞内在化对氧化铁纳米粒子（IONPs）的动态磁响应的影响。交流磁法和两种磁芯尺寸（11和21 nm）的磁化率测量结果强调了细胞摄取后动态磁响应的差异，对于较大尺寸的效应更为明显。已经采用了两种方法来通过实验确定活细胞内磁性纳米颗粒的磁热损失，而又不冒其生存力以及磁性纳米结构对体外热疗研究。我们的实验结果（在理论计算的支持下）表明，细胞内IONP簇的增强主要驱动细胞内在化作用，而不是细胞内IONP固定化。了解与纳米粒子迁移进入活细胞对其磁响应有关的影响，将允许设计包含磁性纳米颗粒的纳米结构，其动态磁响应在任何生物环境中都将保持不变，从而可实现持续且可预测的体内加热效率。
更新日期：2018-03-06
中文翻译：
活细胞内氧化铁纳米粒子的动态磁响应
暴露在交变磁场中的磁性纳米粒子已显示出巨大的潜力，可作为磁高温疗法用于癌症治疗。然而，当纳米颗粒位于细胞或组织内部时，已经证明了纳米颗粒磁加热效率的显着和无法解释的降低。最近的研究表明，与细胞相互作用后，纳米颗粒的聚集和/或固定化的增强是可能的原因，尽管仍然缺乏关于在交流磁场下生物基质对磁性纳米颗粒磁响应的影响的定量描述。在这里，我们研究了细胞内在化对氧化铁纳米粒子（IONPs）的动态磁响应的影响。交流磁法和两种磁芯尺寸（11和21 nm）的磁化率测量结果强调了细胞摄取后动态磁响应的差异，对于较大尺寸的效应更为明显。已经采用了两种方法来通过实验确定活细胞内磁性纳米颗粒的磁热损失，而又不冒其生存力以及磁性纳米结构对体外热疗研究。我们的实验结果（在理论计算的支持下）表明，细胞内IONP簇的增强主要驱动细胞内在化作用，而不是细胞内IONP固定化。了解与纳米粒子迁移进入活细胞对其磁响应有关的影响，将允许设计包含磁性纳米颗粒的纳米结构，其动态磁响应在任何生物环境中都将保持不变，从而可实现持续且可预测的体内加热效率。
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