Insight into the Aggregation Capacity of Anammox Consortia during Reactor Start-Up,Environmental Science & Technology

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Insight into the Aggregation Capacity of Anammox Consortia during Reactor Start-Up
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2018-03-09 00:00:00 , DOI: 10.1021/acs.est.7b06553
Yunpeng Zhao _{1,

2} , Ying Feng _{1,

2} , Jianqi Li _{2,

3} , Yongzhao Guo _{2,

3} , Liming Chen _{1,

2} , Sitong Liu _{1,

2}

Affiliation

Anammox aggregates have been extensively observed in high-efficiency nitrogen-removal reactors, yet the variation and inherent cause of its aggregation capacity related to reactor operation are still unknown. Here, we used microbial detection, metabolomics, extended Derjaguin–Landau–Verwey–Overbeek theory, and multivariate statistical analysis to address this issue. The aggregation capacity of anammox consortia varied periodically during reactor operation, which was determined by the hydrophobic force and the ratio of extracellular protein (PN) to extracellular polysaccharides (PS). Fundamentally, it related to the variation of polysaccharides degradation bacteria abundance and the discrepancy of consortia metabolism. Specifically, the distinguishable up-regulation of the amino acids Phe, Leu, Ala, Thr, Gly, Glu, and Val potentially contributed to the high biosynthesis of extracellular PN. Together with the reduced extracellular PS production that was regulated via the uridine diphosphate (UDP)-N-acetyl-d-glucosamine and UDP-N-acetyl-d-galactosamine pathways, the elevated extracellular PN-to-PS ratio resulted in the obviously increased extracellular hydrophobicity and aggregation capacity. Additionally, the overtly enriched phosphatidylethanolamine biosynthesis pathway was also vital to increasing extracellular hydrophobicity to accelerate aggregation. Understanding aggregation capacity variation is useful for advancing anammox aggregation for its application in wastewater treatment.

中文翻译：

在反应堆启动期间洞悉Anammox联盟的聚集能力

在高效脱氮反应器中已广泛观察到厌氧氨氧化物聚集体，但其与反应堆运行相关的聚集能力的变化和内在原因仍然未知。在这里，我们使用了微生物检测，代谢组学，扩展的Derjaguin–Landau–Verwey–Overbeek理论以及多变量统计分析来解决此问题。厌氧菌团的聚集能力在反应器运行期间周期性变化，这取决于疏水力和细胞外蛋白（PN）与细胞外多糖（PS）的比例。从根本上讲，它与多糖降解细菌丰度的变化和联合体代谢的差异有关。具体而言，氨基酸Phe，Leu，Ala，Thr，Gly，Glu，Val具有促进细胞外PN高生物合成的作用。以及通过尿苷二磷酸（UDP）-调节的细胞外PS生成减少Ñ乙酰基d -葡糖胺和UDP- Ñ乙酰基d -galactosamine途径中，细胞外升高PN到PS比率导致明显增加细胞外的疏水性和聚集能力。另外，明显富集的磷脂酰乙醇胺生物合成途径对增加细胞外疏水性以加速聚集也至关重要。了解聚集能力变化对于推进厌氧氨氧化聚集在废水处理中的应用很有用。

更新日期：2018-03-10

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