Mammalian cytochromes P450 are an important class of enzymes involved in the biotransformation of many endo- and exogenous compounds. Cytochrome P450 isoforms are attached to the membrane of the endoplasmic reticulum or mitochondria, and their catalytic domains move along the membrane surface while being partially immersed in the membrane environment. Their active sites are connected to both the membrane and cytosolic environments via a complex network of access channels. Consequently, they can accept substrates from both environments. The membrane also supports the interactions of cytochromes P450 with their redox partners. In this review, we provide an overview of current knowledge of the structure, flexibility, and interactions with substrates and redox partners of cytochrome P450 on membranes, amalgamating information derived from both experiments and simulations.

哺乳动物细胞色素P450是涉及许多内源性和外源性化合物生物转化的重要一类酶。细胞色素P450同工型附着在内质网或线粒体的膜上，它们的催化结构域沿着膜表面移动，同时部分浸入膜环境中。它们的活性位点通过复杂的通道网络连接到膜和胞质环境。因此，他们可以接受两种环境的基材。该膜还支持细胞色素P450与它们的氧化还原伴侣的相互作用。在本文中，我们概述了细胞色素P450在膜上的结构，柔韧性以及与底物和氧化还原伴侣的相互作用的最新知识，