Few animal models of Zika virus (ZIKV) infection have incorporated arthropod-borne transmission. Here, we establish an Aedes aegypti mosquito model of ZIKV infection of mice, and demonstrate altered vector competency among three strains, (Orlando, ORL, Ho Chi Minh, HCM, and Patilas, PAT). All strains acquired ZIKV in their midguts after a blood meal from infected mice, but ZIKV transmission only occurred in mice fed upon by HCM, and to a lesser extent PAT, but not ORL, mosquitoes. This defect in transmission from ORL or PAT mosquitoes was overcome by intrathoracic injection of ZIKV into mosquito. Genetic analysis revealed significant diversity among these strains, suggesting a genetic basis for differences in ability for mosquito strains to transmit ZIKV. The intrathoracic injection mosquito-mouse transmission model is critical to understanding the influence of mosquitoes on ZIKV transmission, infectivity and pathogenesis in the vertebrate host, and represents a natural transmission route for testing vaccines and therapeutics. (152 words)

寨卡病毒（ZIKV）感染的动物模型很少纳入节肢动物传播的传播方式。在这里，我们建立了埃及伊蚊ZIKV感染小鼠的蚊子模型，并证明了三种品系（奥兰多，ORL，胡志明，HCM和帕提拉斯，PAT）之间载体能力的改变。所有菌株在感染了小鼠的血粉后在其中肠中均获得了ZIKV，但是ZIKV传播仅发生在以HCM喂养的小鼠中，在较小程度上是PAT引起的，而ORL则没有。通过胸腔内注射ZIKV向蚊子克服了ORL或PAT蚊子传播的缺陷。遗传分析显示这些菌株之间存在显着多样性，这为蚊子菌株传播ZIKV的能力差异提供了遗传基础。胸腔内注射蚊鼠传播模型对于了解蚊子对脊椎动物宿主中ZIKV传播，传染性和致病性的影响至关重要，并代表了测试疫苗和治疗剂的自然传播途径。（152个字）