Egg White Ovotransferrin Shows Osteogenic Activity in Osteoblast Cells,Journal of Agricultural and Food Chemistry

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Egg White Ovotransferrin Shows Osteogenic Activity in Osteoblast Cells
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-03-05 00:00:00 , DOI: 10.1021/acs.jafc.8b00069
Nan Shang ₁ , Jianping Wu ₁

Affiliation

Ovotransferrin, the major protein in egg white, is a member of transferrin family. The objective of this study was to study the effects of ovotransferrin on cell proliferation, differentiation, mineralization and osteoclastogenesis of bone osteoblast cells. Effect of ovotransferrin (concentrations ranging from 1 to 1000 μg/mL) on the proliferation, differentiation, and mineralization of mouse osteoblast cells MC3T3-E1 was determined by 5-bromo-2-deoxyuridine (BrdU) incorporation assay, Western blot, immunofluorescence, and Alizarin-S red staining, respectively. Our results showed that ovotransferrin stimulated cell proliferation (enhanced BrdU incorporation), differentiation (enhanced expression of alkaline phosphatase and type-I collagen), and mineralization (increased calcium deposits) in a dose-dependent manner. Furthermore, ovotransferrin could increase the expression of osteoprotegerin (OPG) while decreasing the expression of receptor activator of nuclear factor kappa-B ligand (RANKL), suggesting its role in inhibition of bone resorption. This study demonstrated for the first time that ovotransferrin might promote bone formation while preventing bone resorption, which might open up a new application of egg white protein ovotransferrin as a functional ingredient in bone health management.

中文翻译：

卵清卵转铁蛋白在成骨细胞中显示成骨活性

卵清蛋白是蛋清中的主要蛋白质，是转铁蛋白家族的成员。这项研究的目的是研究卵转铁蛋白对骨成骨细胞的细胞增殖，分化，矿化和破骨细胞生成的影响。通过5-溴-2-脱氧尿苷（BrdU）掺入测定，蛋白质印迹，免疫荧光，免疫荧光法测定卵转铁蛋白（浓度范围为1至1000μg/ mL）对小鼠成骨细胞MC3T3-E1增殖，分化和矿化的影响和茜素-S红色染色分别。我们的结果表明，卵转铁蛋白以剂量依赖的方式刺激细胞增殖（增强BrdU掺入），分化（增强碱性磷酸酶和I型胶原的表达）和矿化（钙沉积增加）。此外，卵转铁蛋白可以增加骨保护素（OPG）的表达，同时降低核因子κB配体（RANKL）受体激活剂的表达，提示其在抑制骨吸收中的作用。这项研究首次证明了卵转铁蛋白可能在促进骨骼形成的同时阻止了骨吸收，这可能开辟了蛋清蛋白卵转铁蛋白作为骨骼健康管理功能成分的新应用。

更新日期：2018-03-05

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