The Maillard Reaction Reduced the Sensitization of Tropomyosin and Arginine Kinase from Scylla paramamosain, Simultaneously,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The Maillard Reaction Reduced the Sensitization of Tropomyosin and Arginine Kinase from Scylla paramamosain, Simultaneously
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-03-02 00:00:00 , DOI: 10.1021/acs.jafc.7b05195
Xin-Yu Han ₁ , Huang Yang ₁ , Shi-Tao Rao ₂ , Guang-Yu Liu ₁ , Meng-Jun Hu ₁ , Bin-Chang Zeng ₁ , Min-Jie Cao ₁ , Guang-Ming Liu ₁

Affiliation

The Maillard reaction was established to reduce the sensitization of tropomyosin (TM) and arginine kinase (AK) from Scylla paramamosain, and the mechanism of the attenuated sensitization was investigated. In the present study, the Maillard reaction conditions were optimized for heating at 100 °C for 60 min (pH 8.5) with arabinose. A low level of allergenicity in mice was shown by the levels of allergen-specific antibodies, and more Th1 and less Th2 cells cytokines produced and associated transcription factors with the Maillard reacted allergen (mAllergen). The tolerance potency in mice was demonstrated by the increased ratio of Th1/Th2 cytokines. Moreover, mass spectrometry analysis showed that some key amino acids of IgE-binding epitopes (K₁₁₂, R₁₂₅, R₁₃₃ of TM; K₃₃, K₁₁₈, R₂₀₂ of AK) were modified by the Maillard reaction. The Maillard reaction with arabinose reduced the sensitization of TM and AK, which may be due to the masked epitopes.

中文翻译：

美拉德反应的减少和原肌球蛋白激酶精氨酸的致敏性青蟹paramamosain，同时

建立了美拉德反应以减少对副飞毛猴的原肌球蛋白（TM）和精氨酸激酶（AK）的敏化，并研究了敏化减弱的机理。在本研究中，对美拉德反应条件进行了优化，可与阿拉伯糖在100°C下加热60分钟（pH 8.5）。过敏原特异性抗体的水平显示了小鼠的低致敏性水平，并且产生了更多的Th1和更少的Th2细胞细胞因子，以及与美拉德反应的过敏原（mAllergen）相关的转录因子。Th1 / Th2细胞因子比例增加证明了小鼠的耐受力。此外，质谱分析表明，IgE结合表位的一些关键氨基酸（K ₁₁₂，R ₁₂₅，RTM ₁₃₃；通过美拉德反应对K ₃₃，K ₁₁₈，AK的R ₂₀₂进行修饰。与阿拉伯糖的美拉德反应降低了TM和AK的敏化作用，这可能是由于被掩盖的表位所致。

更新日期：2018-03-02

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11