Synergy of Two Highly Specific Biomolecular Recognition Events: Aligning an AT-Hook Peptide in DNA Minor Grooves via Covalent Conjugation to 2′-Amino-LNA,Bioconjugate Chemistry

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Synergy of Two Highly Specific Biomolecular Recognition Events: Aligning an AT-Hook Peptide in DNA Minor Grooves via Covalent Conjugation to 2′-Amino-LNA
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-03-05 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00101
Maria Ejlersen ₁ , Niels Johan Christensen ₂ , Kasper K. Sørensen ₂ , Knud J. Jensen ₂ , Jesper Wengel ₁ , Chenguang Lou ₁

Affiliation

Two highly specific biomolecular recognition events, nucleic acid duplex hybridization and DNA-peptide recognition in the minor groove, were coalesced in a miniature ensemble for the first time by covalently attaching a natural AT-hook peptide motif to nucleic acid duplexes via a 2′-amino-LNA scaffold. A combination of molecular dynamics simulations and ultraviolet thermal denaturation studies revealed high sequence-specific affinity of the peptide–oligonucleotide conjugates (POCs) when binding to complementary DNA strands, leveraging the bioinformation encrypted in the minor groove of DNA duplexes. The significant cooperative DNA duplex stabilization may pave the way toward further development of POCs with enhanced affinity and selectivity toward target sequences carrying peptide-binding genetic islands.

中文翻译：

两种高度特异性的生物分子识别事件的协同作用：通过共价结合到2'-氨基-LNA，比对DNA小沟中的AT钩肽

通过将天然AT钩肽基元通过2'-共价连接到核酸双链体上，首次在微型集合中合并了两个高度特异性的生物分子识别事件，即小双沟中的核酸双链体杂交和DNA肽识别。氨基LNA支架。分子动力学模拟和紫外线热变性研究相结合，揭示了与互补DNA链结合时，肽-寡核苷酸共轭物（POC）具有很高的序列特异性亲和力，并利用了在DNA双链体小沟中加密的生物信息。显着的协同DNA双链体稳定作用可以为POC的进一步开发铺平道路，从而进一步提高对带有肽结合遗传岛的靶序列的亲和力和选择性。

更新日期：2018-03-05

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