The protein corona has emerged as an important determinant of biological response in nanoparticle (NP) drug delivery. However, there is presently no reported study on how the protein corona affects the behavior of NPs in microflow and its subsequent interactions with the vascular endothelium, which could affect their delivery to the target tumor site regardless of its targeting mechanism. Furthermore, a consensus on the role of physical and surface characteristics of NPs in affecting the margination of NPs is lacking due to different methods of quantifying margination. In this study, we examine how the particle adhesion (PA) method and particle distribution (PD) method quantify the margination of 20, 40, 100, and 200 nm polystyrene NPs (pNPs) differently in fibronectin or pluronic F-127-coated microfluidic straight channels. We found that PA reduced with increasing pNP size, whereas the PD was similar across all pNP sizes regardless of channel coating. We then formed a protein corona on all pNPs (pNPs-PC) and found that the protein corona increased the adhesion of 40–200 nm pNPs in fibronectin-coated channels, with no size dependence between them except for 40 nm, which had significantly higher particle adhesion. The PA method was also dependent on channel coating, whereas the PD method was independent of channel coating. These results suggested that the PA method was more amenable to surface interactions between the pNPs and the channel wall while providing a measure of the amount of NPs that interacted with the channel walls, whereas the PD method provided a representation of their distribution across the channel due to margination. The two methods complement each other to elucidate a more holistic understanding of how different factors might affect a NP’s margination in future studies.

中文翻译：

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用微流定量电晕蛋白定量分析纳米粒子的血管分布和粘附。

电晕蛋白已成为纳米颗粒（NP）药物递送中生物学反应的重要决定因素。然而，目前尚无关于电晕蛋白如何影响微流中NPs行为及其随后与血管内皮相互作用的报道，无论其靶向机制如何，NP都可能影响其向靶肿瘤部位的递送。此外，由于不同的量化边际方法，人们对NP的物理和表面特征在影响NP边际方面的作用尚缺乏共识。在这项研究中，我们研究了颗粒粘附（PA）方法和颗粒分布（PD）方法如何在纤连蛋白或普朗尼克F-127涂层微流体中以不同的方式量化20、40、100和200 nm聚苯乙烯NP（pNP）的边缘化直渠道。我们发现，PA随pNP尺寸的增加而减小，而PD在所有pNP尺寸上均相似，而与通道涂层无关。然后，我们在所有pNP（pNPs-PC）上形成了蛋白电晕，发现该蛋白电晕增加了纤连蛋白包被通道中40–200 nm pNP的粘附力，除了40 nm以外，它们之间没有大小依赖性，而40 nm明显更高。颗粒附着力。PA方法也依赖于通道涂层，而PD方法则独立于通道涂层。这些结果表明，PA方法更适合pNP与通道壁之间的表面相互作用，同时提供了与通道壁相互作用的NP数量的量度，而PD方法提供了它们在通道中分布的表示。边缘化。