Wounds in the fetus can heal without scarring. Consequently, biomaterials that attempt to recapitulate the biophysical and biochemical properties of fetal skin have emerged as promising pro-regenerative strategies. The extracellular matrix (ECM) protein fibronectin (Fn) in particular is believed to play a crucial role in directing this regenerative phenotype. Accordingly, Fn has been implicated in numerous wound healing studies, yet remains untested in its fibrillar conformation as found in fetal skin. Here, we show that high extensional (∼1.2 $\times$10⁵ s⁻¹) and shear (∼3 $\times$10⁵ s⁻¹) strain rates in rotary jet spinning (RJS) can drive high throughput Fn fibrillogenesis (∼10 mL/min), thus producing nanofiber scaffolds that are used to effectively enhance wound healing. When tested on a full-thickness wound mouse model, Fn nanofiber dressings not only accelerated wound closure, but also significantly improved tissue restoration, recovering dermal and epidermal structures as well as skin appendages and adipose tissue. Together, these results suggest that bioprotein nanofiber fabrication via RJS could set a new paradigm for enhancing wound healing and may thus find use in a variety of regenerative medicine applications.

胎儿的伤口可以愈合而不会留下疤痕。因此，试图概括胎儿皮肤的生物物理和生化特性的生物材料已成为有希望的促再生策略。人们认为，细胞外基质（ECM）蛋白纤连蛋白（Fn）在指导这种再生表型中起着至关重要的作用。因此，Fn已经参与了许多伤口愈合研究，但仍未测试其在胎儿皮肤中发现的纤维状构象。在这里，我们显示出高延展性（〜1.2$\times$10 ⁵ s ^-1）和剪切力（〜3$\times$旋转喷射纺丝（RJS）中的10 ⁵ s ^-1）应变速率可以驱动高通量Fn原纤维生成（〜10 mL / min），从而生产出可有效增强伤口愈合的纳米纤维支架。在全厚度伤口小鼠模型上进行测试时，Fn纳米纤维敷料不仅可以加速伤口闭合，而且可以显着改善组织恢复能力，恢复真皮和表皮结构以及皮肤附件和脂肪组织。总之，这些结果表明，通过RJS制备生物蛋白纳米纤维可以为增强伤口愈合树立新的典范，因此可以在各种再生医学应用中找到用途。