Efficient Detection of Structure and Dynamics in Unlabeled RNAs: The SELOPE Approach.,Chemistry - A European Journal

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Efficient Detection of Structure and Dynamics in Unlabeled RNAs: The SELOPE Approach.
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2018-03-25 , DOI: 10.1002/chem.201800992
Judith Schlagnitweit ₁ , Emilie Steiner ₁ , Hampus Karlsson ₁ , Katja Petzold ₁

Affiliation

The knowledge of structure and dynamics is crucial to explain the function of RNAs. While nuclear magnetic resonance (NMR) is well suited to probe these for complex biomolecules, it requires expensive, isotopically labeled samples, and long measurement times. Here we present SELOPE, a new robust, proton-only NMR method that allows us to obtain site-specific overview of structure and dynamics in an entire RNA molecule using an unlabeled sample. SELOPE simplifies assignment and allows for cost-effective screening of the response of nucleic acids to physiological changes (e.g. ion concentration) or screening of drugs in a high throughput fashion. This single technique allows us to probe an unprecedented range of exchange time scales (the whole μs to ms motion range) with increased sensitivity, surpassing all current experiments to detect chemical exchange. For the first time we could describe an RNA excited state using an unlabeled RNA.

中文翻译：

有效检测未标记 RNA 的结构和动力学：SELOPE 方法。

结构和动力学知识对于解释 RNA 的功能至关重要。虽然核磁共振 (NMR) 非常适合探测这些复杂的生物分子，但它需要昂贵的同位素标记样品和较长的测量时间。在这里，我们介绍 SELOPE，一种新的稳健的纯质子 NMR 方法，使我们能够使用未标记的样品获得整个 RNA 分子的结构和动力学的位点特异性概述。SELOPE 简化了分配，并允许以高通量方式经济有效地筛选核酸对生理变化（例如离子浓度）的响应或筛选药物。这种单一技术使我们能够以更高的灵敏度探测前所未有的交换时间尺度范围（整个微秒到毫秒的运动范围），超越了当前所有检测化学交换的实验。我们第一次可以使用未标记的 RNA 来描述 RNA 激发态。

更新日期：2018-03-25

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