A Paper Sensor Printed with Multifunctional Bio/Nano Materials,Angewandte Chemie International Edition

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A Paper Sensor Printed with Multifunctional Bio/Nano Materials
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-03-15 , DOI: 10.1002/anie.201712903
Christy Y. Hui ₁ , Meng Liu _{1,

2,

3} , Yingfu Li _{1,

2} , John D. Brennan ₁

Affiliation

We report a paper‐based aptasensor platform that uses two reaction zones and a connecting bridge along with printed multifunctional bio/nano materials to achieve molecular recognition and signal amplification. Upon addition of analyte to the first zone, a fluorescently labelled DNA or RNA aptamer is desorbed from printed graphene oxide, rapidly producing an initial fluorescence signal. The released aptamer then flows to the second zone where it reacts with printed reagents to initiate rolling circle amplification, generating DNA amplicons containing a peroxidase‐mimicking DNAzyme, which produces a colorimetric readout that can be read in an equipment‐free manner or with a smartphone. The sensor was demonstrated using an RNA aptamer for adenosine triphosphate (a bacterial marker) and a DNA aptamer for glutamate dehydrogenase (Clostridium difficile marker) with excellent sensitivity and specificity. These targets could be detected in spiked serum or feacal samples, demonstrating the potential for testing clinical samples.

中文翻译：

印有多功能生物/纳米材料的纸张传感器

我们报告了一种基于纸质的aptasensor平台，该平台使用两个反应区和一个连接桥以及印刷的多功能生物/纳米材料来实现分子识别和信号放大。将分析物添加到第一区域后，荧光标记的DNA或RNA适体会从印刷的氧化石墨烯上解吸，从而迅速产生初始荧光信号。然后，释放的适体流到第二个区域，在该区域中它与打印的试剂发生反应，以启动滚环扩增，生成包含模仿过氧化物酶的DNAzyme的DNA扩增子，从而产生比色读数，可以用无设备方式或通过智能手机读取。使用用于三磷酸腺苷的RNA适体（细菌标记）和用于谷氨酸脱氢酶的DNA适体（艰难梭状芽胞杆菌标记）证明了该传感器具有出色的灵敏度和特异性。这些目标可以在加标的血清或粪便样品中检测到，证明了测试临床样品的潜力。

更新日期：2018-03-15

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