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Highly Biocompatible Chlorin e6-Loaded Chitosan Nanoparticles for Improved Photodynamic Cancer Therapy
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-03-02 00:00:00 , DOI: 10.1021/acsami.8b01522 Yuan-Fu Ding 1, 2 , Shengke Li 2 , Lijun Liang 1 , Qiaoxian Huang 2 , Lihui Yuwen 1 , Wenjing Yang 1 , Ruibing Wang 2 , Lian-Hui Wang 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-03-02 00:00:00 , DOI: 10.1021/acsami.8b01522 Yuan-Fu Ding 1, 2 , Shengke Li 2 , Lijun Liang 1 , Qiaoxian Huang 2 , Lihui Yuwen 1 , Wenjing Yang 1 , Ruibing Wang 2 , Lian-Hui Wang 1
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The photosensitizer Chlorin e6 (Ce6) has been frequently employed for photodynamic therapy (PDT) of cancer; however, its nonspecific toxicity has limited its clinical applications. In this study, we prepared chitosan nanoparticles (CNPs), with a mean diameter of approximately 130 nm, by a nonsolvent-aided counterion complexation method in an aqueous solution, into which Ce6 could be physically entrapped during the preparation process. These CNPs and Ce6-loaded CNPs (CNPs-Ce6) were fully characterized by UV–vis, photoluminescence, and Fourier transform infrared spectroscopic analysis, as well as dynamic light scattering and transmission electron microscopy measurements. More importantly, the biocompatibility of the otherwise toxic Ce6 was significantly improved upon its loading into the CNPs, as demonstrated by both confocal laser scanning microscopy analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Furthermore, the PDT efficiency of Ce6-loaded CNPs was dramatically enhanced, in comparison with that of the free Ce6, as shown by both MTT and flow cytometry assays. This discovery provides a novel strategy for improving the biocompatibility and therapeutic efficacy of PDT agents by using a natural, biocompatible polysaccharide carrier.
中文翻译：
高度生物相容性的含氯绿素e6的壳聚糖纳米颗粒，用于改善光动力癌症治疗。
光敏剂Chlorin e6（Ce6）经常用于癌症的光动力疗法（PDT）。然而，其非特异性毒性限制了其临床应用。在这项研究中，我们通过非溶剂辅助的抗衡离子络合方法在水溶液中制备了平均直径约为130 nm的壳聚糖纳米粒子（CNP），在制备过程中可能会物理地捕获Ce6。这些CNP和装载Ce6的CNP（CNPs-Ce6）通过紫外可见，光致发光和傅立叶变换红外光谱分析以及动态光散射和透射电子显微镜测量得到了充分的表征。更重要的是，原本有毒的Ce6加载到CNP中后，其生物相容性得到了显着改善，如通过共聚焦激光扫描显微镜分析和3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）分析所证明。此外，与MtTT和流式细胞术检测结果相比，与游离Ce6相比，负载Ce6的CNP的PDT效率得到了显着提高。该发现为通过使用天然的生物相容性多糖载体改善PDT剂的生物相容性和治疗功效提供了一种新颖的策略。
更新日期：2018-03-02
中文翻译：
高度生物相容性的含氯绿素e6的壳聚糖纳米颗粒，用于改善光动力癌症治疗。
光敏剂Chlorin e6（Ce6）经常用于癌症的光动力疗法（PDT）。然而，其非特异性毒性限制了其临床应用。在这项研究中，我们通过非溶剂辅助的抗衡离子络合方法在水溶液中制备了平均直径约为130 nm的壳聚糖纳米粒子（CNP），在制备过程中可能会物理地捕获Ce6。这些CNP和装载Ce6的CNP（CNPs-Ce6）通过紫外可见，光致发光和傅立叶变换红外光谱分析以及动态光散射和透射电子显微镜测量得到了充分的表征。更重要的是，原本有毒的Ce6加载到CNP中后，其生物相容性得到了显着改善，如通过共聚焦激光扫描显微镜分析和3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）分析所证明。此外，与MtTT和流式细胞术检测结果相比，与游离Ce6相比，负载Ce6的CNP的PDT效率得到了显着提高。该发现为通过使用天然的生物相容性多糖载体改善PDT剂的生物相容性和治疗功效提供了一种新颖的策略。
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