This paper used piezoelectric sensor to study the dysfunction of endothelial cell monolayer barrier caused by inflammatory factors. The biocompatible conductive polymer membrane of pPy[pGlu]-pLys was prepared on the surface of the ITO work electrode to improve the interface between the endothelial cell and the electrode. Both the impedance analysis data and the stable plateau stage of sensor's frequency shift indicated that endothelial cells formed a good monolayer barrier on this polymer surface. The response frequency shifts of lipopolysaccharide (LPS)- and histamine-induced endothelial barrier dysfunction were different, which distinguished their different stimulation mechanism. It provided a valuable analysis method for detecting the endothelial barrier function affected by inflammatory factor, and could further promote the application of piezoelectric sensor in cell biology and toxicology research.

本文利用压电传感器研究了由炎症因子引起的内皮细胞单层屏障功能障碍。在ITO工作电极的表面上制备了pPy [pGlu] -pLys的生物相容性导电聚合物膜，以改善内皮细胞与电极之间的界面。阻抗分析数据和传感器频移的稳定平稳阶段均表明，内皮细胞在该聚合物表面上形成了良好的单层屏障。脂多糖（LPS）和组胺引起的内皮屏障功能障碍的反应频率变化是不同的，从而区分了它们的不同刺激机制。它为检测炎症因子影响的内皮屏障功能提供了有价值的分析方法，