Mesoporous silica nanoparticles (MSNs), the inorganic nanomaterials with stable physicochemical property and versatile surface functional groups, have been increasingly studied and applied in biomedical field as nanocarriers. There are a limited number of reports on their potential applications in clinical dentistry. Herein, we fabricated the fluorescence-labeled MSNs (RITC-NPs) and investigated for the first time their infiltration profiles in the dentinal tubules of extracted permanent teeth prepared by different irrigation approaches. Ethylenediaminetetraacetic acid (EDTA) enabled removal of the smear layer efficiently and exposure of the dentinal tubules on the root canal walls. Confocal scanning laser microscopy showed that the RITC-NPs could accumulate on the root canal walls, and effectively infiltrate into the dentin. In general, the fluorescence intensity in dentin from EDTA-treated group was much higher than that from the ultrasound-treated group, and stronger red fluorescence was observed at the crown and middle sections than the apical one in the EDTA group. The field-emission gun scanning electron microscopy further confirmed the infiltration effects of RITC-NPs in the dentin, through the patent dentinal tubules. The present findings on the infiltration of MSNs in the root canal system may provide a potential approach for further applying MSN-encapsulated antimicrobials in advanced endodontic therapy.

中文翻译：

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齿管中孔二氧化硅纳米粒子的渗透和分布。

具有稳定的物理化学性质和多功能的表面官能团的无机纳米材料介孔二氧化硅纳米粒子（MSNs）已被越来越多地研究并作为纳米载体应用于生物医学领域。关于它们在临床牙科中的潜在应用的报道很少。在这里，我们制造了荧光标记的MSNs（RITC-NPs），并首次研究了它们在不同冲洗方法制备的拔出恒牙的牙本质小管中的渗透特性。乙二胺四乙酸（EDTA）能够有效去除涂片层，并使根管壁上的牙本质小管暴露在外。共聚焦扫描激光显微镜显示，RITC-NPs可以积聚在根管壁上，并有效地渗透到牙本质中。一般来说，EDTA治疗组牙本质的荧光强度远高于超声治疗组，在牙冠和中部的红色荧光强度明显高于EDTA治疗组的顶端。场发射枪扫描电子显微镜通过牙本质小管进一步证实了RITC-NP在牙本质中的渗透作用。目前有关MSNs在根管系统中的浸润的发现可能为进一步在晚期牙髓治疗中应用MSN封装的抗菌剂提供了一种潜在的方法。场发射枪扫描电子显微镜通过牙本质小管进一步证实了RITC-NP在牙本质中的渗透作用。目前有关MSNs在根管系统中的浸润的发现可能为进一步在晚期牙髓治疗中应用MSN封装的抗菌剂提供了一种潜在的方法。场发射枪扫描电子显微镜通过牙本质小管进一步证实了RITC-NP在牙本质中的渗透作用。目前有关MSNs在根管系统中的浸润的发现可能为进一步在晚期牙髓治疗中应用MSN封装的抗菌剂提供了一种潜在的方法。