Unsaponifiable fraction isolated from grape (Vitis vinifera L.) seed oil attenuates oxidative and inflammatory responses in human primary monocytes,Food & Function

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Unsaponifiable fraction isolated from grape (Vitis vinifera L.) seed oil attenuates oxidative and inflammatory responses in human primary monocytes
Food & Function ( IF 5.1 ) Pub Date : 2018-03-01 00:00:00 , DOI: 10.1039/c8fo00063h
Maria C. Millan-Linares _{1,

2,

3,

4,

5} , Beatriz Bermudez _{5,

6,

7,

8,

9} , Maria E. Martin _{5,

6,

7,

8,

9} , Ernesto Muñoz _{2,

3,

4,

5,

10} , Rocio Abia _{2,

3,

4,

5,

10} , Francisco Millan _{1,

2,

3,

4,

5} , Francisco J. G. Muriana _{2,

3,

4,

5,

10} , Sergio Montserrat-de la Paz _{8,

11,

12,

13,

14}

Affiliation

Grape (Vitis vinifera L.) seed has well-known potential for production of oil as a byproduct of winemaking and is a rich source of bioactive compounds. Herein, we report that the unsaponifiable fraction (UF) isolated from grape seed oil (GSO) possesses anti-oxidative and anti-inflammatory properties towards human primary monocytes. The UF isolated from GSO was phytochemically characterized by GC-MS and HPLC. Freshly obtained human monocytes were used to analyse the effects of GSOUF (10–100 μg mL⁻¹) on oxidative and inflammatory responses using FACS analysis, RT-qPCR, and ELISA procedures. GSOUF skewed the monocyte plasticity towards the anti-inflammatory non-classical CD14⁺CD16⁺⁺ monocytes and reduced the inflammatory competence of LPS-treated human primary monocytes diminishing TNF-α, IL-1β, and IL-6 gene expression and secretion. In addition, GSOUF showed a strong reactive oxygen species (ROS)-scavenging activity, reducing significantly nitrite levels with a significant decrease in Nos2 gene expression. Our results suggest that the UF isolated from GSO has significant potential for the management of inflammatory and oxidative conditions and offer novel benefits derived from the consumption of GSO in the prevention of inflammation-related diseases.

中文翻译：

从葡萄中分离（不皂化物馏分葡萄L.）种子油衰减氧化和在人单核细胞主要炎症反应

葡萄（Vitis vinifera L.）种子具有众所周知的潜力，可作为酿酒的副产品生产石油，并且是生物活性化合物的丰富来源。本文中，我们报道从葡萄籽油（GSO）中分离出的不可皂化级分（UF）具有对人原代单核细胞的抗氧化和抗炎特性。从GSO中分离出的超滤通过GC-MS和HPLC进行了植物化学表征。使用FACS分析，RT-qPCR和ELISA方法，将新鲜获得的人单核细胞用于分析GSOUF（10–100μgmL ^-1）对氧化和炎症反应的影响。GSOUF使单核细胞可塑性趋向于抗炎非经典CD14 ⁺ CD16 ⁺⁺单核细胞减少了LPS处理的人原代单核细胞的炎症能力，从而降低了TNF-α，IL-1β和IL-6基因的表达和分泌。此外，GSOUF表现出很强的清除活性氧（ROS）的活性，可显着降低亚硝酸盐水平，并显着降低Nos2基因的表达。我们的结果表明，从GSO中分离出的超滤粉具有控制炎症和氧化性疾病的巨大潜力，并且由于食用GSO可以预防炎症相关疾病，从而提供了新的益处。

更新日期：2018-03-01

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