Dual-mode holographic microscopy imaging platform†,Lab on a Chip

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Dual-mode holographic microscopy imaging platform†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-02-28 00:00:00 , DOI: 10.1039/c7lc01304c
M. Sanz _{1,

2,

3,

4,

5} , J. A. Picazo-Bueno _{1,

2,

3,

4,

5} , J. García _{1,

2,

3,

4,

5} , V. Micó _{1,

2,

3,

4,

5}

Affiliation

We report on a novel layout capable of dual-mode imaging in real time with different magnifications and resolution capabilities in lensless microscopy. The concept is based on wavelength multiplexing for providing two illuminations with different wavefront curvatures: one is collimated, allowing a large field of view (FOV) with a poor resolution limit, and the other is divergent, to achieve a better resolution limit (micron range) over a small FOV. Moreover, our recently reported concept of MISHELF microscopy [M. Sanz, J. Á. Picazo-Bueno, L. Granero, J. García and V. Micó, Sci. Rep., 2017, 7, 43291] is applied to the divergent illumination case, improving the image quality by noise averaging and twin image minimization. The performance of this novel dual-mode lensless microscopy imaging platform is validated through experiments using both static amplitude (resolution test targets) and dynamic complex (flowing microbeads and live sperm cells) samples. The proposed imaging platform represents the background technology for a new generation of lensless microscopes with improved performance and capabilities.

中文翻译：

双模全息显微成像平台†

我们报告了一种新颖的布局，该布局能够在无透镜显微镜中以不同的放大倍率和分辨率进行实时双模成像。该概念基于波长复用，可提供两种具有不同波前曲率的照明：一种是准直的，允许大视野（FOV），分辨率限制较差，另一种是发散的，以实现更好的分辨率限制（微米范围））在较小的FOV上。此外，我们最近报道了MISHELF显微镜的概念[M. Sanz，J.Á。Picazo-Bueno，L。Granero，J。García和V.Micó，科学。众议员。，2017年，7，[43291]应用于发散照明情况，通过噪声平均和最小化双图像来改善图像质量。通过使用静态振幅（分辨率测试目标）和动态复合物（流动的微珠和活精子细胞）样本进行的实验，验证了这种新型双模式无透镜显微成像平台的性能。拟议的成像平台代表了具有改进性能和功能的新一代无透镜显微镜的背景技术。

更新日期：2018-02-28

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