Photomodulating Gene Expression by Using Caged siRNAs with Single‐Aptamer Modification,ChemBioChem

当前位置： X-MOL 学术 › ChemBioChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Photomodulating Gene Expression by Using Caged siRNAs with Single‐Aptamer Modification
ChemBioChem ( IF 2.6 ) Pub Date : 2018-02-28 , DOI: 10.1002/cbic.201700623
Liangliang Zhang ₁ , Changmai Chen ₁ , Xinli Fan ₁ , Xinjing Tang ₁

Affiliation

Light‐activated gene silencing: Caged siRNAs, each modified with a single aptamer (AS1411 or MUC‐1), were rationally designed. With these caged siRNAs, photomodulation both of firefly luciferase and of GFP expression in cells was successfully achieved. Further patterning experiments revealed successful light‐activated spatial regulation of GFP expression with use of the aptamer‐caged siRNAs.

中文翻译：

通过使用单适体修饰的笼状siRNA光调节基因表达

光激活基因沉默：合理设计每个均用单个适体（AS1411或MUC-1）修饰的笼状siRNA。使用这些笼中的siRNA，萤火虫荧光素酶和GFP在细胞中的表达均得到了成功的光调制。进一步的图案化实验表明，使用适体笼装的siRNA成功地光激活了GFP表达的空间调控。

更新日期：2018-02-28

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11