An aptamer@AuNP-modified POSS–polyethylenimine hybrid affinity monolith with a high aptamer coverage density for sensitive and selective recognition of ochratoxin A†,Journal of Materials Chemistry B

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An aptamer@AuNP-modified POSS–polyethylenimine hybrid affinity monolith with a high aptamer coverage density for sensitive and selective recognition of ochratoxin A†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-02-27 00:00:00 , DOI: 10.1039/c7tb03319b
Xia Yu _{1,

2,

3,

4} , Hongliang Song _{1,

2,

3,

4} , Jing Huang _{1,

2,

3,

4} , Yongxuan Chen _{3,

4,

5} , Ming Dai _{3,

4,

5} , Xucong Lin _{1,

2,

3,

4} , Zenghong Xie _{1,

2,

3,

4}

Affiliation

A novel hybrid affinity monolith with a high aptamer coverage density was developed via a highly efficient modification of aptamer@gold nanoparticles (Apt@AuNPs) on a well-controlled 3D skeletal POSS–polyethylenimine (PEI) matrix. The effects of amine reagents and the skeletal structures of the hybrid monoliths on the modification efficiency of Apt@AuNPs were studied, and the resultant Apt@AuNPs@POSS–PEI1200 monolith was proposed with a high coverage density of aptamer of up to 1413 pmol μL⁻¹, which is 4–5 times more than the coverage density when modified with Apt@AuNPs that was reported previously. Characterization including morphology, spectra analysis, mechanical stability, binding capacity and specificity studies was also carried out. Significant specific recognition targeting of ochratoxin A (OTA) was achieved with a detection limit as low as 0.06 ng mL⁻¹. High recoveries of OTA were achieved of 92.6 ± 1.3% to 94.7 ± 1.4% (n = 3), while the cross-reactivity towards analogues such as ochratoxin B (OTB) and aflatoxin B₁ (AFB₁) was weak. This highlights a facile approach to POSS-based affinity monoliths with a high aptamer coverage density for sensitive and selective recognition of a trace target (OTA).

中文翻译：

aptamer @ AuNP修饰的POSS-聚乙烯亚胺杂合亲和性整体物，具有高适体覆盖密度，可敏感和选择性地识别曲霉毒素A †

通过在一个可控的3D骨骼POSS-聚乙烯亚胺（PEI）基质上对aptamer @ gold纳米粒子（Apt @ AuNPs）进行高效修饰，开发了一种具有高适体覆盖密度的新型杂合亲和整体。研究了胺试剂和杂化整体结构的骨架结构对Apt @ AuNPs修饰效率的影响，并提出了合成的Apt @ AuNPs @ POSS–PEI1200整体结构，其适体覆盖密度高达1413 pmolμL。^-1，这是以前报道的Apt @ AuNPs修饰后的覆盖密度的4-5倍。还进行了表征，包括形态，光谱分析，机械稳定性，结合能力和特异性研究。曲霉毒素A（OTA）的特异性识别目标明显，检出限低至0.06 ng mL ^-1。OTA的高回收率达到92.6±1.3％至94.7±1.4％（n = 3），而与类似物如曲霉毒素B（OTB）和黄曲霉毒素B ₁（AFB ₁）的交叉反应性较弱。这突显了一种基于POSS的亲和整体的简便方法，其具有高适体覆盖密度，可对痕量目标（OTA）进行灵敏和选择性的识别。

更新日期：2018-02-27

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