Cocaine is one of the most used illegal recreational drugs. Developing an on-site test for cocaine use detection has been a focus of research effort, since it is essential to the control and legal action against drug abuse. Currently most of cocaine detection methods are time-consuming and require special or expensive equipment, and the detection often suffers from high cross-reactivity with cocaine metabolites and relative low sensitivity with the best limit of detection reported at sub nanomolar (nM) level. In this work, an aptasensor has been developed using capacitive monitoring of sensor surface incorporating alternating current electrokinetics effects to speed up molecular transport and minimize matrix effects. The aptasensor is rapid, low cost, highly sensitive and specific as well as simple-to-use for the detection of cocaine from serum. The assay has a sample-to-result time of 30 s, a limit of detection of 7.8 fM, and a linear response for cocaine ranging from 14.5fM to 14.5pM in standard buffer, which are great improvements from other reported cocaine sensors. Special buffer is used for serum cocaine detection, and a limit of detection of 13.4 fM is experimentally demonstrated for cocaine spiked in human serum (equivalent to 1.34pM cocaine in neat serum). The specificity of the biosensor is also demonstrated with structurally similar chemicals, ecgonine ethyl ester and methylecgonidine. This biosensor shows high promise in detection of low levels of cocaine from complex matrices.

可卡因是最常用的非法娱乐药物之一。开发可卡因使用检测的现场测试一直是研究工作的重点，因为这对于防止滥用药物的控制和法律行动至关重要。当前，大多数可卡因检测方法耗时且需要专用或昂贵的设备，并且检测经常遭受与可卡因代谢物的高交叉反应性和相对较低的灵敏度，在亚纳摩尔（nM）级别报道的最佳检测限。在这项工作中，使用传感器表面的电容监测技术开发了一种适体传感器，该传感器结合了交流电动力学效应，从而加快了分子传输速度并使基质效应最小化。aptasensor快速，低成本，高度灵敏且特异，并且易于使用，可从血清中检测可卡因。该方法的样品至结果时间为30 s，检测极限为7.8 fM，在标准缓冲液中对可卡因的线性响应范围为14.5fM至14.5pM，与其他已报道的可卡因传感器相比有很大改进。特殊缓冲液用于血清可卡因检测，并且实验证明对加标在人血清中的可卡因（相当于纯血清中的1.34pM可卡因）的检出限为13.4 fM。生物传感器的特异性还可以通过结构相似的化学药品，芽子碱乙酯和甲基芽孢子丁来证明。这种生物传感器在检测复杂基质中的低水平可卡因方面显示出很高的前景。与其他已报告的可卡因传感器相比，有了很大的改进。特殊缓冲液用于血清可卡因检测，并且实验证明了加标到人血清中的可卡因的检测限为13.4 fM（相当于纯血清中的1.34pM可卡因）。生物传感器的特异性还可以通过结构相似的化学药品，芽子碱乙酯和甲基芽孢子丁来证明。这种生物传感器在检测复杂基质中的低水平可卡因方面显示出很高的前景。与其他已报告的可卡因传感器相比，有了很大的改进。特殊缓冲液用于血清可卡因检测，并且实验证明对加标在人血清中的可卡因（相当于纯血清中的1.34pM可卡因）的检出限为13.4 fM。生物传感器的特异性还可以通过结构相似的化学药品，芽子碱乙酯和甲基芽孢子丁来证明。这种生物传感器在检测复杂基质中的低水平可卡因方面显示出很高的前景。