In vitro digestion assays simulate the physiological conditions of digestion in vivo and are useful tools for studying and understanding changes, interactions, as well as the bioaccessibility of nutrients, drugs and non-nutritive compounds. The technique is widely used in fields such as nutrition, pharmacology and food chemistry. Over the last 40 years, more than 2500 research articles have been published using in vitro digestion assays (85% of which have been published in the last two decades) to elucidate multiple aspects such as protein digestibility, nutrient interactions or the viability of encapsulated microorganisms. The most recent trend in the use of this technique involves the determination of the antioxidant activity of bioactive compounds after digestion. However, the inability to reproduce certain in vivo digestion events, as well as the multiple models of in vitro digestion, point to a need to optimize and validate the method with in vivo assays to determine its limitations and uses. The purpose of this paper is to provide an overview of the current state of the art of in vitro digestion models through an analysis of how they have evolved in terms of the development of digestion models (parameters, protocols, guidance) and taking into consideration the boom in new fields of application.

体外消化测定法模拟了体内消化的生理条件，是研究和理解营养素，药物和非营养性化合物的变化，相互作用以及生物可及性的有用工具。该技术广泛用于营养，药理和食品化学等领域。在过去的40年中，使用体外方法发表了2500多篇研究文章消化测定（其中近85％已在最近的二十年中发表）阐明了多个方面，例如蛋白质的消化率，养分相互作用或被包封的微生物的生存能力。使用该技术的最新趋势涉及确定消化后生物活性化合物的抗氧化活性。但是，无法重现某些体内消化事件，以及体外消化的多种模型，都表明需要通过体内试验来优化和验证该方法，以确定其局限性和用途。本文的目的是概述体外技术的最新状况 通过对消化模型在消化模型（参数，协议，指南）的发展方面的发展进行分析，并考虑新应用领域的兴旺发展，从而对消化模型进行分析。