An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy†,Physical Chemistry Chemical Physics

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An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy†
Physical Chemistry Chemical Physics ( IF 2.9 ) Pub Date : 2018-02-26 00:00:00 , DOI: 10.1039/c7cp08478a
D. Xie _{1,

2,

3,

4,

5} , W. F. Zhu _{1,

2,

3,

4} , H. Cheng _{1,

2,

3,

4} , Z. Y. Yao _{1,

2,

3,

4} , M. Li _{1,

2,

3,

4} , Y. L. Zhao _{1,

2,

3,

4,

6}

Affiliation

A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I₁₅₇₄/I₁₅₈₆ for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.

中文翻译：

通过表面增强拉曼光谱同时捕获和检测糖蛋白的无抗体检测方法†

通过表面增强拉曼散射（SERS），在4-巯基苯基硼酸（4-MPBA）修饰的银纳米颗粒薄膜（AgNF）上，提出了无抗体模式下糖蛋白的灵敏且一步反应的测定方法。4-MPBA / AgNF被设计为糖蛋白特异性识别生物传感器和SERS平台的拉曼报告基因。响应糖蛋白捕获，4-MPBA / AgNF的比率I ₁₅₇₄ / I _1586的变化构成了SERS分析，对非糖蛋白表现出高灵敏度和选择性，并在复杂样品中发挥作用。

更新日期：2018-02-26

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