Genetic Code Expansion of the Silkworm Bombyx mori to Functionalize Silk Fiber,ACS Synthetic Biology

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Genetic Code Expansion of the Silkworm Bombyx mori to Functionalize Silk Fiber
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-02-26 00:00:00 , DOI: 10.1021/acssynbio.7b00437
Hidetoshi Teramoto ₁ , Yoshimi Amano ₂ , Fumie Iraha ₂ , Katsura Kojima ₁ , Takuhiro Ito ₂ , Kensaku Sakamoto ₂

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The genetic code in bacteria and animal cells has been expanded to incorporate novel amino acids into proteins. Recent efforts have enabled genetic code expansion in nematodes, flies, and mice, whereas such engineering is rare with industrially useful animals. In the present study, we engineered the silkworm Bombyx mori to synthesize silk fiber functionalized with azidophenylalanine. For this purpose, we developed a bacterial system to screen for B. mori phenylalanyl-tRNA synthetases with altered amino-acid specificity. We created four transgenic B. mori lines expressing the selected synthetase variants in silk glands, and found that two of them supported the efficient in vivo incorporation of azidophenylalanine into silk fiber. The obtained silk was bio-orthogonally reactive with fluorescent molecules. The results showed that genetic code expansion in an industrial animal can be facilitated by prior bacterial selection, to accelerate the development of silk fiber with novel properties.

中文翻译：

家蚕的遗传密码扩展以功能化蚕丝纤维

细菌和动物细胞中的遗传密码已得到扩展，可以将新的氨基酸整合到蛋白质中。最近的努力使线虫，苍蝇和小鼠的遗传密码得以扩展，而这种工程改造在工业上有用的动物中很少见。在本研究中，我们设计了家蚕，以合成用叠氮苯丙氨酸功能化的蚕丝纤维。为了这个目的，我们开发了一种细菌系统，以筛选具有改变的氨基酸特异性的桑蚕苯丙氨酰-tRNA合成酶。我们创建了四个在蚕丝腺中表达所选合成酶变异体的转基因桑蚕线粒体，发现其中两个支持有效的体内将叠氮苯丙氨酸掺入丝纤维中。所获得的丝与荧光分子具有生物正交反应性。结果表明，通过事先进行细菌筛选，可以促进工业动物遗传密码的扩展，从而加速具有新特性的丝纤维的发展。

更新日期：2018-02-26

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