Controllable Assembly of Enzymes for Multiplexed Lab‐on‐a‐Chip Bioassays with a Tunable Detection Range,Angewandte Chemie International Edition

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Controllable Assembly of Enzymes for Multiplexed Lab‐on‐a‐Chip Bioassays with a Tunable Detection Range
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-03-23 , DOI: 10.1002/anie.201801815
Yunlei Xianyu _{1,

2} , Jing Wu _{1,

3} , Yiping Chen ₁ , Wenshu Zheng ₁ , Mengxia Xie ₃ , Xingyu Jiang _{1,

2}

Affiliation

Multiplexed analysis of molecules with different concentrations requires assays with a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in a controlled fashion to generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly of enzymes on detection antibodies allows for lab‐on‐a‐chip immunoassays with a tunable detection range from pg mL⁻¹ to μg mL⁻¹. Simultaneous, multiplexed bioassays of clinically relevant inflammatory biomarkers in serum are demonstrated in one lab‐on‐a‐chip format, with a limit of detection of 0.47 pg mL⁻¹ for interleukin‐6, 2.6 pg mL⁻¹ for procalcitonin, and 40 ng mL⁻¹ for C‐reactive protein. This controlled assembly technique provides a multiplexed platform for simultaneous and quantitative analyses of both low‐abundance and high‐abundance biomarkers with a broad detection range, which holds great promise as a point‐of‐care platform for biomedical diagnostics.

中文翻译：

具有可调检测范围的多种芯片实验室生物测定的酶的可控组装

对具有不同浓度的分子进行多重分析需要检测范围可调的测定。概述了一种策略，该策略使用单击化学以受控方式组装辣根过氧化物酶，以生成酶组装体，作为多重生物测定的探针。检测抗体上酶的这种可控组装允许进行芯片实验室免疫分析，检测范围从pg mL ^-1到μgmL ^-1可调。同时，在血清临床相关炎症标志物的多重生物测定中演示了一个实验室上的单芯片格式，具有检测0.47皮克毫升的限制^-1为白细胞介素-6，2.6皮克毫升^-1对降钙素原，和40 ng毫升^-1用于C反应蛋白这种受控的组装技术为同时检测和定量分析具有广泛检测范围的低丰度和高丰度生物标志物提供了一个多路复用的平台，作为生物医学诊断的即时服务平台，具有广阔的前景。

更新日期：2018-03-23

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