Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins†,CrystEngComm

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Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins†
CrystEngComm ( IF 2.6 ) Pub Date : 2018-02-24 00:00:00 , DOI: 10.1039/c7ce02189e
Ren-Bin Zhou _{1,

2,

3,

4} , Xiao-Li Lu _{1,

2,

3,

4} , Chen Dong _{1,

2,

3,

4} , Fiaz Ahmad _{1,

2,

3,

4} , Chen-Yan Zhang _{1,

2,

3,

4} , Da-Chuan Yin _{1,

2,

3,

4}

Affiliation

Protein crystallization screens are commonly used to find specific crystallization conditions. In practice, undesirable clear drops are often ignored. We hypothesize that clear drops may be potentially useful for finding the conditions leading to proteins with high solubility and monodispersity . Based on this idea, we first tested three model proteins and confirmed that improved monodispersity could be observed in the clear drops. Next, we used this strategy to achieve the maximum solubility and monodispersity of a new protein (RNA binding motif 3, RBM3). We suggest that this strategy is useful not only for high-throughput screening of optimal solubilization conditions but also for screening of biopharmaceutical formulations.

中文翻译：

蛋白质结晶方法的应用，以增强蛋白质的溶解性，稳定性和单分散性†

蛋白质结晶筛选通常用于查找特定的结晶条件。在实践中，通常会忽略不希望有的透明液滴。我们假设清晰的液滴可能对发现导致高溶解度和单分散性蛋白质的条件有用。基于此想法，我们首先测试了三种模型蛋白，并确认可以在透明液滴中观察到改善的单分散性。接下来，我们使用这种策略来实现新蛋白（RNA结合基序3，RBM3）的最大溶解度和单分散性。我们建议该策略不仅对最佳增溶条件的高通量筛选有用，而且对生物药物制剂的筛选也有用。

更新日期：2018-02-24

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